RIP2 Contributes to Expanded CD4 + T Cell IFN-γ Production during Efferocytosis of Streptococcus pneumoniae -Infected Apoptotic Cells.

Autor:	Niño-Castaño VE; Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil.; Department of Pathology, Faculty of Health Science, Universidad del Cauca, Popayán, Cauca, Colombia., Penteado LA; Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil.; Basic and Applied Immunology Program, Ribeirao Preto Medical School, University of Sao Paulo, Sao Paulo, Brazil., Silva-Pereira L; Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil., Bazzano JMR; Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil., Orlando AB; Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil., Salina ACG; Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil.; Basic and Applied Immunology Program, Ribeirao Preto Medical School, University of Sao Paulo, Sao Paulo, Brazil., Dejani NN; Basic and Applied Immunology Program, Ribeirao Preto Medical School, University of Sao Paulo, Sao Paulo, Brazil.; Department of Physiology and Pathology, Federal University of Paraíba, João Pessoa, Paraíba, Brazil., Bonato VLD; Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of Sao Paulo, Sao Paulo, Brazil; and., Serezani CH; Department of Medicine, Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN., Medeiros AI; Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil; alexandra.medeiros@unesp.br.
Jazyk:	angličtina
Zdroj:	ImmunoHorizons [Immunohorizons] 2022 Jul 26; Vol. 6 (7), pp. 559-568. Date of Electronic Publication: 2022 Jul 26.
DOI:	10.4049/immunohorizons.2200001
Abstrakt:	Apoptotic cell clearance by professional and nonprofessional phagocytes in the process of efferocytosis is critical to preserve tissue homeostasis. Uptake of apoptotic cells by dendritic cells generates regulatory T cells and induces immunologic tolerance against self-antigens. In contrast, ingestion of infected apoptotic cells promotes activation of TLR4/MyD88-dependent bone marrow-derived dendritic cells (BMDCs) and triggers Th17 cell differentiation. In this study, we evaluated the impact of Streptococcus pneumoniae -infected apoptotic cell efferocytosis by BMDCs derived from C57BL/6 mice on differentiation and expansion of CD4 + T cell subsets, as well as the role of TLR2/4 and receptor-interacting protein 2 (RIP2) receptors in recognizing intracellular pathogens during efferocytosis. We demonstrated that BMDC-mediated efferocytosis of S. pneumoniae -infected apoptotic cells induced Th1 cell differentiation and expansion. Although TLR2/4 and RIP2 deficiency in BMDCs did not affect Th1 cell differentiation during efferocytosis, the absence of RIP2 decreased IFN-γ production by CD4 T cells during the expansion phase. These findings suggest that RIP2-mediated IL-1β production during efferocytosis of S. pneumoniae -infected apoptotic cells partially supports a Th1-mediated IFN-γ production microenvironment. (Copyright © 2022 The Authors.)
Databáze:	MEDLINE
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