Non-invasive skin sampling detects systemically administered drugs in humans.

Autor: Panitchpakdi M; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America.; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, United States of America., Weldon KC; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America.; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, United States of America.; Center for Microbiome Innovation, University of California, San Diego, La Jolla, California, United States of America., Jarmusch AK; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America.; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, United States of America.; Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, United States of America., Gentry EC; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America.; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, United States of America., Choi A; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America., Sepulveda Y; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America., Aguirre S; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America.; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, United States of America., Sun K; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America.; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, United States of America., Momper JD; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America., Dorrestein PC; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America.; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, United States of America.; Department of Pediatrics, School of Medicine, University of California, San Diego, La Jolla, California, United States of America., Tsunoda SM; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Jul 26; Vol. 17 (7), pp. e0271794. Date of Electronic Publication: 2022 Jul 26 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0271794
Abstrakt: Clinical testing typically relies on invasive blood draws and biopsies. Alternative methods of sample collection are continually being developed to improve patient experience; swabbing the skin is one of the least invasive sampling methods possible. To show that skin swabs in combination with untargeted mass spectrometry (metabolomics) can be used for non-invasive monitoring of an oral drug, we report the kinetics and metabolism of diphenhydramine in healthy volunteers (n = 10) over the course of 24 hours in blood and three regions of the skin. Diphenhydramine and its metabolites were observed on the skin after peak plasma levels, varying by compound and skin location, and is an illustrative example of how systemically administered molecules can be detected on the skin surface. The observation of diphenhydramine directly from the skin supports the hypothesis that both parent drug and metabolites can be qualitatively measured from a simple non-invasive swab of the skin surface. The mechanism of the drug and metabolites pathway to the skin's surface remains unknown.
Competing Interests: P.C.D is a scientific advisor to Sirenas, Galileo and Cybele and co-founder and scientific advisor to Ometa Labs and Enveda with approval by the University of California San Diego. M.P. is a research consultant to Ometa Labs.
Databáze: MEDLINE
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