Assessing Antigen Presentation on the Surface of Plasmodium falciparum-Infected Erythrocytes by Photoactivated Localization Microscopy (PALM).

Autor:	Karathanasis C; Institute for Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany., Sanchez CP; Center of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, Heidelberg, Germany., Heilemann M; Institute for Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany.; BioQuant-Center for Quantitative Biology, Heidelberg University, Heidelberg, Germany., Lanzer M; Center of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, Heidelberg, Germany. michael.lanzer@med.uni-heidelberg.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2470, pp. 457-466.
DOI:	10.1007/978-1-0716-2189-9_34
Abstrakt:	Super-resolution microscopy in the form of photoactivated localization microscopy (PALM) offers the possibility of counting single molecules in a cell, a cellular compartment or a molecular complex. PALM can, therefore, underpin molecular and biochemical processes with a numeric and stoichiometric understanding of the interacting players. Here, we introduce the physical principles underlying PALM and provide a step-by-step protocol of how to apply PALM to questions related to the biology and pathophysiology of P. falciparum and other malaria parasites. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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