Protocol for Differential Biopanning of P. falciparum Phage Display cDNA Library to Identify Parasite Targets of Protective Antibodies.
| Autor: | Zuromski J; Department of Pathology and Laboratory Medicine, Brown University Medical School, Providence, RI, USA., Kurtis J; Department of Pathology and Laboratory Medicine, Brown University Medical School, Providence, RI, USA., Raj DK; Department of Pathology and Laboratory Medicine, Brown University Medical School, Providence, RI, USA. Dipak_Raj@Brown.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2470, pp. 359-366. |
| DOI: | 10.1007/978-1-0716-2189-9_26 |
| Abstrakt: | Malaria remains a significant global health burden, killing hundreds of thousands of children annually (WHO, The world malaria report. WHO, Geneva, 2019). Despite decades of effort, no broadly effective vaccine exists. Differential screening of parasite phage display libraries is a promising approach to identify the targets of human antibodies expressed by resistant but not by susceptible individuals (Raj et al., Nature, 582, 104-108, 2020; Science, 344, 871-877, 2014). Our whole proteome differential screening (WPDS) approach consists of positive selection to capture phage that bind antibodies expressed by malaria-resistant individuals, followed by negative selection to remove phage that bind antibodies expressed by malaria-susceptible individuals, and amplification of differentially recognized clones. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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