Accuracy of Allplex SARS-CoV-2 assay amplification curve analysis for the detection of SARS-CoV-2 variant Alpha.

Autor:	Čretnik TŽ; National Laboratory of Health, Environment & Food, Maribor, Slovenia., Retelj M; National Laboratory of Health, Environment & Food, Maribor, Slovenia., Janežič S; National Laboratory of Health, Environment & Food, Maribor, Slovenia.; Faculty of Medicine, University of Maribor, Maribor, Slovenia., Mahnič A; National Laboratory of Health, Environment & Food, Maribor, Slovenia., Tesovnik T; Department of Paediatric Endocrinology, Diabetes & Metabolism, University Children's Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia.; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia., Šket R; Department of Paediatric Endocrinology, Diabetes & Metabolism, University Children's Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia.; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia., Bizjan BJ; Department of Paediatric Endocrinology, Diabetes & Metabolism, University Children's Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia.; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia., Jeverica S; National Laboratory of Health, Environment & Food, Maribor, Slovenia., Ravnik M; National Laboratory of Health, Environment & Food, Maribor, Slovenia., Rak M; National Laboratory of Health, Environment & Food, Maribor, Slovenia., Borinc M; National Laboratory of Health, Environment & Food, Maribor, Slovenia., Rupnik M; National Laboratory of Health, Environment & Food, Maribor, Slovenia.; Faculty of Medicine, University of Maribor, Maribor, Slovenia., Battelino T; Department of Paediatric Endocrinology, Diabetes & Metabolism, University Children's Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia.; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia., Pokorn M; Department of Paediatric Endocrinology, Diabetes & Metabolism, University Children's Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia.; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia., Kovač J; Department of Paediatric Endocrinology, Diabetes & Metabolism, University Children's Hospital, University Medical Centre Ljubljana, Ljubljana, Slovenia.; Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Jazyk:	angličtina
Zdroj:	Future microbiology [Future Microbiol] 2022 Sep; Vol. 17, pp. 1125-1131. Date of Electronic Publication: 2022 Jul 26.
DOI:	10.2217/fmb-2021-0288
Abstrakt:	Aim: To evaluate the accuracy of two PCR-based techniques for detecting SARS-CoV-2 variant Alpha (B.1.1.7). Materials & methods: A multicenter prospective cohort with 1137 positive specimens from Slovenia was studied. A mutation-based assay (rTEST-COVID-19 qPCR B.1.1.7 assay) and amplification curve pattern analysis of the Allplex SARS-CoV-2 assay were compared with whole-genome sequencing. Results: SARS-CoV-2 variant Alpha was detected in 155 samples (13.6%). Sensitivity and specificity were 98.1 and 98.0%, respectively, for the rTEST-COVID-19 qPCR B.1.1.7 assay and 97.4 and 97.5%, respectively, for amplification curve pattern analysis. Conclusion: The good analytical performance of both methods was confirmed for the preliminary identification of SARS-CoV-2 variant Alpha. This cost-effective principle for screening SARS-CoV-2 populations is also applicable to other emerging variants and may help to conserve some whole-genome sequencing resources.
Databáze:	MEDLINE
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