| Autor: |
Lee I; Department of Life Science, Multidisciplinary Genome Institute, Hallym University, Chuncheon 24252, Republic of Korea.; Institute for Liver and Digestive Diseases, Hallym University, Chuncheon, Republic of Korea., Podolich O; Institute of Molecular Biology and Genetics of NASU, Kyiv 03143, Ukraine., Brenig B; Institute of Veterinary Medicine, Burckhardtweg, University of Göttingen, Göttingen 37073, Germany., Tiwari S; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 6627, Brazil., Azevedo V; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 6627, Brazil., de Carvalho DS; Molecular and Computational Biology of Fungi Laboratory, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 6627, Brazil., Uetanabaro APT; Molecular and Computational Biology of Fungi Laboratory, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 6627, Brazil., Góes-Neto A; Molecular and Computational Biology of Fungi Laboratory, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 6627, Brazil., Alzahrani KJ; Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, Taif 21944, Saudi Arabia., Reva O; Centre for Bioinformatics and Computational Biology, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0028, South Africa., Kozyrovska N; Institute of Molecular Biology and Genetics of NASU, Kyiv 03143, Ukraine., de Vera JP; Microgravity User Support Center (MUSC), German Aerospace Center (DLR), Cologne 51147, Germany., Barh D; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 6627, Brazil.; Institute of Integrative Omics and Applied Biotechnology (IIOAB), Nonakuri, Purba Medinipur, WB, 721172, India., Kim BS; Department of Life Science, Multidisciplinary Genome Institute, Hallym University, Chuncheon 24252, Republic of Korea.; The Korean Institute of Nutrition, Hallym University, Chuncheon 24252, Republic of Korea. |
| Abstrakt: |
Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (MAGs) of the Komagataeibacter , which is a predominant genus in KMC, to understand their roles in the KMC after exposure to Mars-like conditions (outside the International Space Station) based on functional genetic elements. We constructed three MAGs: K. hansenii , K. rhaeticus , and K. oboediens . Our results showed that (i) K. oboediens MAG functionally more complex than K. hansenii , (ii) K. hansenii is a keystone in KMCs with specific functional features to tolerate extreme stress, and (iii) genes related to the PPDK, betaine biosynthesis, polyamines biosynthesis, sulfate-sulfur assimilation pathway as well as type II toxin-antitoxin (TA) system, quorum sensing (QS) system, and cellulose production could play important roles in the resilience of KMC after exposure to Mars-like stress. Our findings show the potential mechanisms through which Komagataeibacter tolerates the extraterrestrial stress and will help to understand minimal microbial composition of KMC for space travelers. |
