Hyphomonas sediminis sp. nov., isolated from marine sediment.

Autor: Wang L; Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, 201198, People's Republic of China., He W; Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, 201198, People's Republic of China., Gao ZY; Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, 201198, People's Republic of China., Zhang DF; Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, 201198, People's Republic of China., Huang JK; Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, 201198, People's Republic of China., Liu C; Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, 201198, People's Republic of China., Li WJ; Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, 201198, People's Republic of China. liwenjun3@mail.sysu.edu.cn.; State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China. liwenjun3@mail.sysu.edu.cn.; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, People's Republic of China. liwenjun3@mail.sysu.edu.cn., Zhang AH; Institute of Marine Biotechnology and Bio-Resource Utilization, College of Oceanography, Hohai University, Nanjing, 201198, People's Republic of China. ahz@hhu.edu.cn.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2022 Sep; Vol. 115 (9), pp. 1177-1185. Date of Electronic Publication: 2022 Jul 25.
DOI: 10.1007/s10482-022-01765-1
Abstrakt: A Gram-staining-negative, aerobic and pear-shaped bacterial strain, designated WL0036 T , was isolated from coastal sediment sample collected in Nantong city, Jiangsu province of China (120° 51' 13″ E, 32° 6' 26″ N) in October 2020. Strain WL0036 T was found to grow at 20-37 °C (optimum, 28 °C) with 0-9.0% NaCl (optimum, 2.5-4.0%) and displayed alkaliphilic growth with the pH range of pH 6.0-10.0 (optimum, pH 7.0-8.0). The polar lipids profile of strain WL0036 T included phosphatidylcholine, phosphatidylethanolamine, glycolipid and an unidentified lipid. The major isoprenoid quinone was determined to be Q-11 and the major fatty acids were C 16:0 , 11-methyl-C 18:1 ω7c, and summed features 8 (C 18:1 ω6c and/or C 18:1 ω7c). The G + C content of genomic DNA was 61.8%. Phylogenetic trees constructed based on 16S rRNA gene sequence and bac120 gene set (a collection of 120 single-copy protein sequences prevalent in bacteria) indicted that strain WL0036 T clustered with strains Hyphomonas neptunium ATCC 15444 T and H. polymorpha PS728 T . The average nucleotide identities between strain WL0036 T and strains H. neptunium ATCC 15444 T and H. polymorpha PS728 T were 80.7% and 81.2%, respectively. Strain WL0036 T showed 22.8% and 23.2% of digital DNA-DNA hybridization identities with H. neptunium ATCC 15444 T and H. polymorpha PS728 T , respectively. As inferred from the phenotypic and genotypic characteristics and the phylogenetic trees, strain WL0036 T ought to be recognized as a novel species in genus Hyphomonas, for which the name Hyphomonas sediminis sp. nov. is proposed. The type strain is WL0036 T (= MCCC 1K05843 T  = JCM 34658 T  = GDMCC 1.2413 T ).
(© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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