| Autor: |
Brüggemann Y; Department of Molecular and Medical Virology, Ruhr University Bochum, Bochum, Germany., Kratzel A; Institute of Virology and Immunology (IVI), Bern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland., Almeida L; Institute of Virology and Immunology (IVI), Bern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland., Kelly JN; Institute of Virology and Immunology (IVI), Bern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; European Virus Bioinformatics Center, Jena, Germany.; Multidisciplinary Center for Infectious Diseases, University of Bern, Bern, Switzerland., Thiel V; Institute of Virology and Immunology (IVI), Bern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; European Virus Bioinformatics Center, Jena, Germany.; Multidisciplinary Center for Infectious Diseases, University of Bern, Bern, Switzerland., Pfaender S; Department of Molecular and Medical Virology, Ruhr University Bochum, Bochum, Germany. |
| Abstrakt: |
The recurrence of zoonotic transmission events highlights the need for novel treatment strategies against emerging coronaviruses (CoVs), namely SARS-CoV, MERS-CoV and most notably SARS-CoV-2. Our recently performed genome-wide CRISPR knockout screen revealed a list of conserved pan-coronavirus as well as MERS-CoV or HCoV-229E-specific host dependency factors (HDF) essential during the viral life cycle. Intriguingly, we identified the macroautophagy/autophagy pathway-regulating immunophilins FKBP8, TMEM41B, and MINAR1 as conserved MERS-CoV, HCoV-229E, SARS-CoV, and SARS-CoV-2 host factors, which further constitute potential targets for therapeutic intervention by clinically approved drugs. |
