A Novel Neuraminidase Virus-Like Particle Vaccine Offers Protection Against Heterologous H3N2 Influenza Virus Infection in the Porcine Model.

Autor:	Pliasas VC; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States., Menne Z; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States.; Department of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, GA, United States., Aida V; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States., Yin JH; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States., Naskou MC; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States., Neasham PJ; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States., North JF; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States., Wilson D; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States., Horzmann KA; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States., Jacob J; Department of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, GA, United States., Skountzou I; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States.; Department of Microbiology and Immunology, School of Medicine, Emory University, Atlanta, GA, United States., Kyriakis CS; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States.; Center for Vaccines and Immunology, University of Georgia, Athens, GA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2022 Jul 07; Vol. 13, pp. 915364. Date of Electronic Publication: 2022 Jul 07 (Print Publication: 2022).
DOI:	10.3389/fimmu.2022.915364
Abstrakt:	Influenza A viruses (IAVs) pose a global health threat, contributing to hundreds of thousands of deaths and millions of hospitalizations annually. The two major surface glycoproteins of IAVs, hemagglutinin (HA) and neuraminidase (NA), are important antigens in eliciting neutralizing antibodies and protection against disease. However, NA is generally ignored in the formulation and development of influenza vaccines. In this study, we evaluate the immunogenicity and efficacy against challenge of a novel NA virus-like particles (VLPs) vaccine in the porcine model. We developed an NA2 VLP vaccine containing the NA protein from A/Perth/16/2009 (H3N2) and the matrix 1 (M1) protein from A/MI/73/2015, formulated with a water-in-oil-in-water adjuvant. Responses to NA2 VLPs were compared to a commercial adjuvanted quadrivalent whole inactivated virus (QWIV) swine IAV vaccine. Animals were prime boost vaccinated 21 days apart and challenged four weeks later with an H3N2 swine IAV field isolate, A/swine/NC/KH1552516/2016. Pigs vaccinated with the commercial QWIV vaccine demonstrated high hemagglutination inhibition (HAI) titers but very weak anti-NA antibody titers and subsequently undetectable NA inhibition (NAI) titers. Conversely, NA2 VLP vaccinated pigs demonstrated undetectable HAI titers but high anti-NA antibody titers and NAI titers. Post-challenge, NA2 VLPs and the commercial QWIV vaccine showed similar reductions in virus replication, pulmonary neutrophilic infiltration, and lung inflammation compared to unvaccinated controls. These data suggest that anti-NA immunity following NA2 VLP vaccination offers comparable protection to QWIV swine IAV vaccines inducing primarily anti-HA responses. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Pliasas, Menne, Aida, Yin, Naskou, Neasham, North, Wilson, Horzmann, Jacob, Skountzou and Kyriakis.)
Databáze:	MEDLINE
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