Preparation and Characterisation of Liposomes of Bergenia Ciliata Extract and Evaluation of their Hepatoprotective Activity.

Autor:	Latief U; Centre for Basic and Translational Research in Health Sciences, Guru Nanak Dev University, Amritsar, 143005, India., Kaur M; Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, 143005, India., Dar SH; Department of Chemistry, Jamia Millia Islamia, New Delhi, 110025, India., Thakur S; Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, 143005, India., Per TS; Department of Botany, Govt. Degree College Doda, Jammu and Kashmir, 182202, India., Tung GK; Centre for Basic and Translational Research in Health Sciences, Guru Nanak Dev University, Amritsar, 143005, India., Jain SK; Centre for Basic and Translational Research in Health Sciences, Guru Nanak Dev University, Amritsar, 143005, India; Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, 143005, India. Electronic address: subheetjain@rediffmail.com.
Jazyk:	angličtina
Zdroj:	Journal of pharmaceutical sciences [J Pharm Sci] 2023 Jan; Vol. 112 (1), pp. 328-335. Date of Electronic Publication: 2022 Jul 21.
DOI:	10.1016/j.xphs.2022.07.013
Abstrakt:	Acute liver damage (ALD) can cause biochemical and pathological changes, which can lead to major complications and even death. The goal of the study was to examine the therapeutic efficacy of liposomes of Bergenia ciliata extract against thioacetamide-induced liver damage in rats. Liposomal batches of B. ciliata extract were prepared by altering the kind and amount of phospholipids and characterized through various physiochemical properties such as laser diffraction, TEM, encapsulation efficiency, stability and in-vitro release studies. In-vivo hepatoprotective studies were performed on TAA-induced acute hepatic damage model. Further, in-silico studies of bergenin against the three hepatic damage markers viz. TGF-β1, TNF-α and interleukin-6 were also performed. Laser diffraction and TEM showed that most stable liposome batch of B. ciliata extract were in the range of 678-1170 nm with encapsulation efficiency of 84.3±3.5. Extract was found to be rapidly dissociated from B. ciliata liposomes in HCl than PBS, according to in-vitro release data. In-vivo data revealed a significant decline in LFT indicators, amelioration of pathological changes and high bergenin bioavailability in the liposomal group. Protective activity of bergenin against ALD targets like TGF-β1, TNF-α and interleukin-6 was anticipated via molecular docking research. As a result, the current findings of the study indicate that B. ciliata liposomes and bergenin have promising ameliorative potential in the management of ALD. Competing Interests: Declaration of Competing Interest The authors declare that there is no conflict of interest. (Copyright © 2022 American Pharmacists Association. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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