Ameliorative effect of Spirulina and Saccharomyces cerevisiae against fipronil toxicity in Oreochromis niloticus.

Autor: Fadl SE; Biochemistry Department, Faculty of Veterinary Medicine - Matrouh University, Matrouh 51744, Egypt. Electronic address: nourmallak@mau.edu.eg., Elbialy ZI; Fish Processing and Biotechnology Department, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh 33516, Egypt. Electronic address: zizyelbialy74@gmail.com., Abdo W; Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Shaikh 33516, Egypt. Electronic address: walid.eid@vet.kfs.edu.eg., Saad AH; Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Matrouh University, Matrouh 51744, Egypt. Electronic address: adelhassan129@gmail.com., Aboubakr M; Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt. Electronic address: mohamed.aboubakr@fvtm.bu.edu.eg., Abdeen A; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt. Electronic address: ahmed.abdeen@fvtm.bu.edu.eg., Elkamshishi MM; Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt. Electronic address: elkamshishymohamed@yahoo.com., Salah AS; Faculty of Aquatic and Fisheries Sciences, Department of Aquaculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt. Electronic address: Abdallah_salah_2014@fsh.kfs.edu.eg., El-Mleeh A; Department of Pharmacology, Faculty of Veterinary Medicine, Menoufia University, Shebin Elkoum 32514, Egypt. Electronic address: amany.ahmed1074@gmail.com., Almeer R; Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. Electronic address: ralmeer@ksu.edu.sa., Aleya L; Chrono-Environnement Laboratory, UMR CNRS 6249, Bourgogne, Franche-Comté University, CEDEX, F-25030 Besançon, France. Electronic address: lotfi.aleya@univ-fcomte.fr., Abdel-Daim MM; Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. Electronic address: abdeldaim.m@vet.suez.edu.eg., Najda A; Department of Vegetable Crops and Medicinal Plants University of Life Sciences in Lublin, 50A Doświadczalna Street, 20-280 Lublin, Poland. Electronic address: agnieszka.najda@up.lublin.pl., Abdelhiee EY; Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Matrouh University, Matrouh 51744, Egypt. Electronic address: ehabyahya76@mau.edu.eg.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2022 Sep 01; Vol. 242, pp. 113899. Date of Electronic Publication: 2022 Jul 20.
DOI: 10.1016/j.ecoenv.2022.113899
Abstrakt: The ameliorative effects of Spirulina and Saccharomyces cerevisiae (S. cerevisiae) against fipronil toxicity in Nile tilapia fish were investigated. Fipronil is a kind of pesticide that is widely used in agriculture, thus this trial was conducted to evaluate the effect of fipronil on growth related parameters (final body weight, feed intake, weight gain, feed conversion ratio, specific growth rate, and protein efficiency ratio), hematology related parameters (RBCs, WBCs, hemoglobin, packed cell volume, and deferential leukocytic count), biochemistry related parameters (alanine aminotransferase, aspartate aminotransferase, total protein, albumin, urea, and creatinine), histopathology of liver, intestine, gills, and spleen, and gene expression of antioxidants, stress, inflammatory, apoptotic, and related to junction proteins genes as SOD and GPx, COX II, TNF-α, Casp-3, and Claudin-3, respectively, in Nile tilapia (Oreochromis niloticus). Four hundred and five Nile tilapia fish were distributed in a glass aquarium into nine groups according to the Spirulina and S. cerevisiae supplemented diets, with or without fipronil contaminated water. The classified groups are control, Sc: S. cerevisiae (4 g/Kg diet), Sp: Spirulina (1 g/100 g diet), Fb1: 0.0021 mg fipronil/L, ScFb1: S. cerevisiae (4 g/Kg diet) with 0.0021 mg fipronil/L, SpFb1: Spirulina (1 g/100 g diet) with 0.0021 mg fipronil/L, Fb2: 0.0042 mg fipronil/L, ScFb2: S. cerevisiae (4 g/Kg diet) with 0.0042 mg fipronil/L, and SpFb2: Spirulina (1 g/100 g diet) with 0.0042 mg fipronil/L. The results of the present investigation indicated the negative effect of fipronil on the growth performance parameters of Nile tilapia, which was confirmed by the results of hematology, biochemistry, and histopathology. In addition, the results of gene expression of antioxidants, stress, inflammatory, and apoptotic genes indicate the genotoxicity of fipronil. However, these negative effects were ameliorated by Spirulina and Saccharomyces dietary supplementation.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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