Fluxes of the Amazon River plume nutrients and microbes into marine sponges.

Autor: de Menezes TA; Laboratory of Microbiology, Biology Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., de Freitas MAM; Laboratory of Microbiology, Biology Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Lima MS; Laboratory of Microbiology, Biology Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Soares AC; Bioinformatics Laboratory, Department of Biochemistry, Institute of Chemistry, University of São Paulo (USP), São Paulo, Brazil., Leal C; Laboratory of Microbiology, Biology Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Busch MS; Laboratory of Lipids Biochemistry and Lipoprotein, Biochemistry Institute Leopoldo de Meis, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Tschoeke DA; Biomedical Engineering Program - COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., de O Vidal L; Environmental Sciences Laboratory, Biosciences and Biotechnology Center, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil; Department of Ecology and Marine Resources, Institute of Biosciences, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil., Atella GC; Laboratory of Lipids Biochemistry and Lipoprotein, Biochemistry Institute Leopoldo de Meis, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Kruger RH; Laboratory of Enzymology, University of Brasilia (UNB), Brasilia, Brazil., Setubal J; Bioinformatics Laboratory, Department of Biochemistry, Institute of Chemistry, University of São Paulo (USP), São Paulo, Brazil., Vasconcelos AA; Greenpeace Brazil, Rio de Janeiro, Brazil., de Mahiques MM; Oceanographic Institute (IO), University of São Paulo (USP), São Paulo, Brazil., Siegle E; Oceanographic Institute (IO), University of São Paulo (USP), São Paulo, Brazil., Asp NE; Federal University of Pará, Institute of Coastal Studies (IECOS), Bragança Campus, Bragança, PA, Brazil., Cosenza C; Center of Technology - CT2, SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Hajdu E; Department of Invertebrates, National Museum, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., de Rezende CE; Environmental Sciences Laboratory, Biosciences and Biotechnology Center, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brazil. Electronic address: crezende@uenf.br., Thompson CC; Laboratory of Microbiology, Biology Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. Electronic address: thompsoncristiane@gmail.com., Thompson FL; Laboratory of Microbiology, Biology Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil; Center of Technology - CT2, SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. Electronic address: fabianothompson1@gmail.com.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2022 Nov 15; Vol. 847, pp. 157474. Date of Electronic Publication: 2022 Jul 20.
DOI: 10.1016/j.scitotenv.2022.157474
Abstrakt: Sponges have co-evolved with microbes for over 400 myr. Previous studies have demonstrated that sponges can be classified according to the abundance of microbes in their tissues as Low Microbial Abundance (LMA) and High Microbial Abundance (HMA). While LMA sponges rely mainly on water column microbes, HMA appear to rely much more on symbiotic fermentative and autotrophic microbes maintained in their tissues. However, it is unclear if this pattern holds when comparing different species of tropical sponges under extreme nutrient conditions and sediment loads in the water column, such as the Great Amazon Reef System (GARS), which covers an area of ~56,000 km 2 off the Amazon River mouth. Sponges are the major GARS benthic components. However, these sponges' microbiome across the GARS is still unknown. Here, we investigated water quality, isotopic values (δ 13 C and δ 15 N), metagenomic and lipidomic profiles of sponges obtained from different sectors throughout the GARS. >180 million shotgun metagenomic reads were annotated, covering 22 sponge species. Isotopic and lipidomic analyses suggested LMA sponges rely on the Amazon River Plume for nutrition. HMA sponges (N = 15) had higher Roseiflexus and Nitrospira abundance, whereas LMA sponges (N = 7) had higher Prochlorococcus and Pelagibacter abundance. Functional data revealed that the LMA sponge microbiomes had greater number of sequences related to phages and prophages as well as electron transport and photophosphorylation which may be related to photosynthetic processes associated with the Prochlorococcus and Synechococcus found in the LMA. The higher phages abundance in LMA sponges could be related to these holobionts' reduced defense towards phage infection. Meanwhile, HMA sponge microbiomes had higher Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR abundance, which may be involved in defense against phage infection. This study sheds light on the nutrient fluxes and microbes from the Amazon River plume into the sponge holobionts.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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