A New Triterpenoid Saponin from Albizia zygia Induced Apoptosis by Reduction of Mitochondrial Potential Status in Malignant Melanoma Cells.

Autor:	Simo LM; Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé I, Yaoundé, Cameroun., Messi LM; Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé I, Yaoundé, Cameroun.; Laboratoire Régional de Lutte Biologique et de Microbiologie Appliquée, Institut de Recherche Agricole pour le Développement, Yaoundé, Cameroun., Mbing JN; Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé I, Yaoundé, Cameroun., Muller CD; UMR 7213 CNRS, Laboratoire de Biophotonique et Pharmacologie, Université de Strasbourg, Faculté de Pharmacie, Strasbourg, France., Boyom FF; Antimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon., Begoudé AB; Laboratoire Régional de Lutte Biologique et de Microbiologie Appliquée, Institut de Recherche Agricole pour le Développement, Yaoundé, Cameroun., Pegnyemb DE; Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé I, Yaoundé, Cameroun., Haddad M; UMR 152 Pharma Dev, Université de Toulouse, IRD, UPS, Toulouse, France., Noté OP; Laboratoire de Pharmacochimie des Substances Naturelles, Département de Chimie Organique, Faculté de Sciences, Université de Yaoundé I, Yaoundé, Cameroun.
Jazyk:	angličtina
Zdroj:	Planta medica [Planta Med] 2023 Jan; Vol. 89 (1), pp. 86-98. Date of Electronic Publication: 2022 Jul 22.
DOI:	10.1055/a-1806-2692
Abstrakt:	In our ongoing research program on the proapoptotic function of saponins, two previously undescribed saponins, named zygiaosides E (1: ) and F (2: ), were isolated from the leaves of Albizia zygia . Their structures were established based on extensive analysis of 1D and 2D NMR data, HR-ESI-MS analysis, and by chemical degradation. The proapoptotic effect of zygiaoside E (1: ) was evaluated on human malignant melanoma (A375), human epidermoid cancer (A431), and normal Homo sapiens skin tissue (TE 353.SK.) cell lines by cytometric analysis. Zygiaoside E (1: ) induced apoptosis of the two human cancer cell lines (A375 and A431) in a dose-dependent manner at 1 µM but did not induce apoptosis in noncancerous skin cells (TE 353.Sk), even when treated with concentrations up to 15 µM. The underlying mechanism of the apoptosis induction activity of zygiaoside E (1: ) on the mitochondrial membrane potential status in A375 cells was further assessed by monitoring the uptake rate of DiOC6, a mitochondrial specific and voltage-dependent fluorescent dye. The number of malignant melanoma cells emitting high fluorescence levels was decreased when cells were treated with 3 or 5 µM of zygiaoside E (1: ) during either 12 or 24 h, thereby revealing a drop of mitochondrial membrane potential in A375 cells upon treatment, which indicated mitochondrial perturbation. Competing Interests: The authors declare that they have no conflict of interest. The findings described herein are a consolidation of independent research carried out in the laboratories of the authors. As such, none of its content in any shape or form is deemed liable to any other individual or organization. (Thieme. All rights reserved.)
Databáze:	MEDLINE
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