Plant genetic effects on microbial hubs impact host fitness in repeated field trials.

Autor:	Brachi B; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637.; University of Bordeaux, INRAE, BIOGECO, F-33610 Cestas, France., Filiault D; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, 1030 Vienna, Austria., Whitehurst H; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Darme P; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Le Gars P; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Le Mentec M; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Morton TC; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Kerdaffrec E; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, 1030 Vienna, Austria., Rabanal F; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, 1030 Vienna, Austria., Anastasio A; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Box MS; Gene in the Environment, John Innes Center, Norwich, NR47UH, United Kingdom., Duncan S; Gene in the Environment, John Innes Center, Norwich, NR47UH, United Kingdom., Huang F; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637.; South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510520, China., Leff R; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Novikova P; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, 1030 Vienna, Austria., Perisin M; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Tsuchimatsu T; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, 1030 Vienna, Austria., Woolley R; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637., Dean C; Gene in the Environment, John Innes Center, Norwich, NR47UH, United Kingdom., Nordborg M; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, 1030 Vienna, Austria., Holm S; Department of Natural Sciences, Mid-Sweden University, HLV SE-851 Sundsvall, Sweden., Bergelson J; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637.; Center for Genomics and System Biology, Department of Biology, New York University, New York, NY, 10003.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Jul 26; Vol. 119 (30), pp. e2201285119. Date of Electronic Publication: 2022 Jul 22.
DOI:	10.1073/pnas.2201285119
Abstrakt:	Although complex interactions between hosts and microbial associates are increasingly well documented, we still know little about how and why hosts shape microbial communities in nature. In addition, host genetic effects on microbial communities vary widely depending on the environment, obscuring conclusions about which microbes are impacted and which plant functions are important. We characterized the leaf microbiota of 200 Arabidopsis thaliana genotypes in eight field experiments and detected consistent host effects on specific, broadly distributed microbial species (operational taxonomic unit [OTUs]). Host genetic effects disproportionately influenced central ecological hubs, with heritability of particular OTUs declining with their distance from the nearest hub within the microbial network. These host effects could reflect either OTUs preferentially associating with specific genotypes or differential microbial success within them. Host genetics associated with microbial hubs explained over 10% of the variation in lifetime seed production among host genotypes across sites and years. We successfully cultured one of these microbial hubs and demonstrated its growth-promoting effects on plants in sterile conditions. Finally, genome-wide association mapping identified many putatively causal genes with small effects on the relative abundance of microbial hubs across sites and years, and these genes were enriched for those involved in the synthesis of specialized metabolites, auxins, and the immune system. Using untargeted metabolomics, we corroborate the consistent association between variation in specialized metabolites and microbial hubs across field sites. Together, our results reveal that host genetic variation impacts the microbial communities in consistent ways across environments and that these effects contribute to fitness variation among host genotypes.
Databáze:	MEDLINE
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