Involvement of a neutrophil-mast cell axis in the effects of Piper malacophyllum (C. PESL) C. DC extract and its isolated compounds in a mouse model of dysmenorrhoea.

Autor: Quintão NLM; Biomedicine Course, School of Health Sciences, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil.; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil.; Pharmacy Course, School of Health Sciences, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil., Reis JP; Biological Science Course, School of See, Science and Technology, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil., Benvenutti L; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., Nunes R; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., Goldoni FC; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., Cozer MS; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., de Souza P; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., de Cássia Melo Vilhena de Andrade Fonseca da Silva R; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., Melato J; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., Vaz CR; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., Whitaker JCP; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., Jesuíno FW; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil., Costa MC; Pharmacy Course, School of Health Sciences, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil., Pastor MVD; Biomedicine Course, School of Health Sciences, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil., Malheiros A; Biomedicine Course, School of Health Sciences, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil.; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil.; Pharmacy Course, School of Health Sciences, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil., Meyre-Silva C; Postgraduate Program of Pharmacy, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil., Santin JR; Biomedicine Course, School of Health Sciences, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil. jrs.santin@univali.br.; Postgraduate Program in Pharmaceutical Science, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, 88302-901, Brazil. jrs.santin@univali.br.; Pharmacy Course, School of Health Sciences, Universidade Do Vale Do Itajaí (UNIVALI), Itajaí, SC, Brazil. jrs.santin@univali.br.
Jazyk: angličtina
Zdroj: Inflammopharmacology [Inflammopharmacology] 2022 Dec; Vol. 30 (6), pp. 2489-2504. Date of Electronic Publication: 2022 Jul 22.
DOI: 10.1007/s10787-022-01032-9
Abstrakt: The effects of Piper malacophyllum (C. Pesl) C. DC extracts and its isolated compounds were analysed in a mouse model of primary dysmenorrhoea (PD). Female Swiss mice (6-8 weeks old) on proestrus were intraperitoneally treated with estradiol benzoate for 3 days, to induce PD. Twenty-four hours later, animals were treated 24 h later with vehicle, plant extract, gibbilimbol B, 4,6-dimethoxy-5-E-phenylbutenolide, mixture of 4,6-dimethoxy-5-E-phenylbutenolide and 4,6-dimethoxy-5-Z-phenylbutenolide, or ibuprofen. One hour later, oxytocin was injected and the numbers of abdominal writhing were counted. Then, mice were euthanized and uteri were collected for morphometrical and histological analyses. The effects of P. malacophyllum in inflammation were investigated in mouse peritoneal neutrophils culture stimulated with LPS or fMLP (chemotaxis and mediator release). Finally, uterus contractile and relaxing responses were assessed. Similar to ibuprofen, P. malacophyllum extract and isolated compounds reduced abdominal writhing in mice with PD. Histology indicated a marked neutrophil and mast cell infiltrate in the uterus of PD animals which was attenuated by the extract. The compounds and the extract reduced neutrophil chemotaxis and inflammatory mediator release by these cells. Reduced TNF levels were also observed in uteri of PD mice treated with P. malacophyllum. The extract did not affect spontaneous uterine contractions nor those induced by carbachol or KCl. However, it caused relaxation of oxytocin-induced uterine contraction, an effect blunted by H1 receptor antagonist. Overall the results indicate that P. malacophyllum may represent interesting natural tools for reliving PD symptoms, reducing the triad of pain, inflammation and spasmodic uterus behaviour.
(© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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