Genetic structure of some candidate genes of repeat breeder syndrome in Egyptian buffaloes.

Autor: Mahrous KF; Cell Biology Department, National Research Centre, Giza, Egypt. l_fathy@yahoo.com., El-Kader HAMA; Cell Biology Department, National Research Centre, Giza, Egypt., Abdelhafez MA; Cell Biology Department, National Research Centre, Giza, Egypt., Aboelenin MM; Cell Biology Department, National Research Centre, Giza, Egypt., Balabel EA; Cell Biology Department, National Research Centre, Giza, Egypt., Mabrouk DM; Cell Biology Department, National Research Centre, Giza, Egypt., El Malky OM; Animal Production Research Institute, Agricultural Research Center, and Ministry of Agriculture, Dokki, Egypt., Hassanane MS; Cell Biology Department, National Research Centre, Giza, Egypt.
Jazyk: angličtina
Zdroj: Journal, genetic engineering & biotechnology [J Genet Eng Biotechnol] 2022 Jul 22; Vol. 20 (1), pp. 110. Date of Electronic Publication: 2022 Jul 22.
DOI: 10.1186/s43141-022-00397-2
Abstrakt: Background: This study aimed to explore the association between polymorphisms in three genes: leptin (LEP), leptin receptor (LEPR), and BMP4, and incidence of repeat breeding in Egyptian buffaloes.
Methods: DNA was extracted from 160 female buffaloes, involving 108 fertile and 52 repeat breeders. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Sequence analysis and alignment were performed by employing NCBI/BLAST/blastn suite, to identify SNPs among different patterns and alleles. We utilized PredictSNP software to predict the non-synonymous SNPs influences on protein function. Moreover, the conservation score of the amino acids within the target proteins was computed by ConSurf server.
Results: The genotyping results showed that LEP and BMP4 genes were monomorphic (CC, GG) in all tested fertile and repeat breeder buffaloes. Leptin gene sequencing showed a non-synonymous C73T SNP, replacing R to C at position 25 within the leptin polypeptide (position 4 in the mature form; R4C) which is a neutral mutation, not affecting function or structure of LEP protein. For LEPR, one synonymous SNP (T102C) and two non-synonymous SNPs (A106G and C146A), triggering V967A and G954C replacements, respectively in LEPR protein. Moreover, they are neutral mutations. Sequencing results of BMP4 showed HinfI restriction site indicate fixed GG genotype (CC genotype in the anti-sense strand) in all sequenced samples. No SNPs were observed within the amplified region.
Conclusion: Genotyping and sequencing results of the surveyed three genes revealed that there is no association between these genes mutations and the incidence of repeat breeding in Egyptian buffaloes.
(© 2022. The Author(s).)
Databáze: MEDLINE
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