Modular micro-PCR system for the onsite rapid diagnosis of COVID-19.

Autor: Nguyen PQM; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore.; Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Block 4, #04-08, Singapore, 117583 Singapore., Wang M; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore., Ann Maria N; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore., Li AY; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore., Tan HY; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore., Xiong GM; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore., Tan MM; Advanced MedTech, 2 Venture Drive, #23-18 Vision Exchange, Singapore, 608526 Singapore., Bhagat AAS; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore.; Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Block 4, #04-08, Singapore, 117583 Singapore., Ong CWM; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore.; Infectious Diseases Translational Research Programme, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, 1E Kent Ridge Road Level 11, Singapore, 119228 Singapore., Lim CT; Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, MD6, 14 Medical Drive #14-01, Singapore, 117599 Singapore.; Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Block 4, #04-08, Singapore, 117583 Singapore.
Jazyk: angličtina
Zdroj: Microsystems & nanoengineering [Microsyst Nanoeng] 2022 Jul 19; Vol. 8, pp. 82. Date of Electronic Publication: 2022 Jul 19 (Print Publication: 2022).
DOI: 10.1038/s41378-022-00400-3
Abstrakt: Effective containment of the COVID-19 pandemic requires rapid and accurate detection of the pathogen. Polymerase chain reaction (PCR) remains the gold standard for COVID-19 confirmation. In this article, we report the performance of a cost-effective modular microfluidic reverse transcription (RT)-PCR and RT-loop mediated isothermal amplification (RT-LAMP) platform, Epidax®, for the point-of-care testing and confirmation of SARS-CoV-2. This platform is versatile and can be reconfigured either for screening using endpoint RT-PCR or RT-LAMP tests or for confirmatory tests using real-time RT-PCR. Epidax® is highly sensitive and detects as little as 1 RNA copy per µL for real-time and endpoint RT-PCR, while using only half of the reagents. We achieved comparable results with those of a commercial platform when detecting SARS-CoV-2 viruses from 81 clinical RNA extracts. Epidax® can also detect SARS-CoV-2 from 44 nasopharyngeal samples without RNA extraction by using a direct RT-PCR assay, which shortens the sample-to-answer time to an hour with minimal user steps. Furthermore, we validated the technology using an RT-LAMP assay on 54 clinical RNA extracts. Overall, our platform provides a sensitive, cost-effective, and accurate diagnostic solution for low-resource settings.
Competing Interests: Conflict of interestC.T.L., P.Q.M.N., M.W., A.Y.L., G.M.X., and A.A.S.B., along with others, have filed a patent application on the technology described here.
(© The Author(s) 2022.)
Databáze: MEDLINE
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