Post-Transcriptional Dynamics is Involved in Rapid Adaptation to Hypergravity in Jurkat T Cells.

Autor:	Vahlensieck C; Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland.; Innovation Cluster Space and Aviation (UZH Space Hub), Air Force Center, University of Zurich, Dübendorf, Switzerland., Thiel CS; Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland.; Innovation Cluster Space and Aviation (UZH Space Hub), Air Force Center, University of Zurich, Dübendorf, Switzerland.; Space Life Sciences Laboratory (SLSL), Kennedy Space Center (KSC), Merritt Island, FL, United States.; Space Biotechnology, Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany., Pöschl D; Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland., Bradley T; Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland., Krammer S; Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland.; Innovation Cluster Space and Aviation (UZH Space Hub), Air Force Center, University of Zurich, Dübendorf, Switzerland., Lauber B; Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland., Polzer J; Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland., Ullrich O; Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland.; Innovation Cluster Space and Aviation (UZH Space Hub), Air Force Center, University of Zurich, Dübendorf, Switzerland.; Space Life Sciences Laboratory (SLSL), Kennedy Space Center (KSC), Merritt Island, FL, United States.; Space Biotechnology, Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany.; Space Medicine, Ernst-Abbe-Hochschule (EAH) Jena, Department of Industrial Engineering, Jena, Germany.; Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland.
Jazyk:	angličtina
Zdroj:	Frontiers in cell and developmental biology [Front Cell Dev Biol] 2022 Jul 04; Vol. 10, pp. 933984. Date of Electronic Publication: 2022 Jul 04 (Print Publication: 2022).
DOI:	10.3389/fcell.2022.933984
Abstrakt:	The transcriptome of human immune cells rapidly reacts to altered gravity in a highly dynamic way. We could show in previous experiments that transcriptional patterns show profound adaption after seconds to minutes of altered gravity. To gain further insight into these transcriptional alteration and adaption dynamics, we conducted a highly standardized RNA-Seq experiment with human Jurkat T cells exposed to 9xg hypergravity for 3 and 15 min, respectively. We investigated the frequency with which individual exons were used during transcription and discovered that differential exon usage broadly appeared after 3 min and became less pronounced after 15 min. Additionally, we observed a shift in the transcript pool from coding towards non-coding transcripts. Thus, adaption of gravity-sensitive differentially expressed genes followed a dynamic transcriptional rebound effect. The general dynamics were compatible with previous studies on the transcriptional effects of short hypergravity on human immune cells and suggest that initial up-regulatory changes mostly result from increased elongation rates. The shift correlated with a general downregulation of the affected genes. All chromosome bands carried homogenous numbers of gravity-sensitive genes but showed a specific tendency towards up- or downregulation. Altered gravity affected transcriptional regulation throughout the entire genome, whereby the direction of differential expression was strongly dependent on the structural location in the genome. A correlation analysis with potential mediators of the early transcriptional response identified a link between initially upregulated genes with certain transcription factors. Based on these findings, we have been able to further develop our model of the transcriptional response to altered gravity. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Vahlensieck, Thiel, Pöschl, Bradley, Krammer, Lauber, Polzer and Ullrich.)
Databáze:	MEDLINE
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