Virulence, antimicrobial susceptibility and phylogenetic analysis of Cronobacter sakazakii isolates of food origins from Jordan.

Autor: Jaradat ZW; Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan., Al-Mousa WA; Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan., Elbetieha AM; Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan., Ababneh QO; Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan., Al-Nabulsi AA; Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid, Jordan., Jang H; US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Laurel, Maryland, USA., Gangiredla J; US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Laurel, Maryland, USA., Patel IR; US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Laurel, Maryland, USA., Gopinath GR; US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Laurel, Maryland, USA., Tall BD; US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Laurel, Maryland, USA.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2022 Oct; Vol. 133 (4), pp. 2528-2546. Date of Electronic Publication: 2022 Aug 05.
DOI: 10.1111/jam.15723
Abstrakt: Aims: The aim was to characterize a collection of Cronobacter sakazakii isolates collected from various origins in Jordan.
Methods and Results: The isolates were characterized using 16S rRNA sequencing, DNA microarray, multi-locus sequence typing (MLST), O-serotyping, virulence gene identification and antibiotic susceptibility testing. The identities and phylogenetic relatedness revealed that C. sakazakii sequence type 4 (ST4) and Csak O:1 serotype were the most prevalent STs and serovars amongst these C. sakazakii strains. PCR screening of putative virulence genes showed that the siderophore-interacting protein gene (sip) and iron acquisition gene clusters (eitCBAD and iucABCD/iutA) were the most detected genes with noticeable variability in the type 6 secretion system (T6SS) and filamentous hemagglutinin/adhesion (FHA) gene loci. The antibiotic resistance profiles revealed that the majority of the isolates were susceptible to all antibiotics used despite harbouring a class C β-lactamase resistance gene.
Conclusions: The results described in this report provide additional insights about the considerable genotypic and phenotypic heterogeneity within C. sakazakii.
Significance and Impact of the Study: The information reported in this study might be of great value in understanding the origins of C. sakazakii isolates, in addition to their diversity and variability, which might be helpful in preventing future outbreaks of this pathogen.
(© 2022 Society for Applied Microbiology.)
Databáze: MEDLINE
Externí odkaz: