Rhodococcus pseudokoreensis sp. nov. isolated from the rhizosphere of young M26 apple rootstocks.

Autor: Kämpfer P; Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, 35392, Giessen, Germany. peter.kaempfer@umwelt.uni-giessen.de., Glaeser SP; Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, 35392, Giessen, Germany., Blom J; Bioinformatics and Systems Biology, Giessen, 35392, Giessen, Germany., Wolf J; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, 38124, Brunswick, Germany., Benning S; Research Unit for Comparative Microbiome Analysis, Helmholtz Zentrum Muenchen - National Research Center for Environmental Health, Oberschleissheim, 85758, Munich, Germany., Schloter M; Research Unit for Comparative Microbiome Analysis, Helmholtz Zentrum Muenchen - National Research Center for Environmental Health, Oberschleissheim, 85758, Munich, Germany., Neumann-Schaal M; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, 38124, Brunswick, Germany.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2022 Jul 20; Vol. 204 (8), pp. 505. Date of Electronic Publication: 2022 Jul 20.
DOI: 10.1007/s00203-022-03079-2
Abstrakt: The Gram-positive strain R79 T , isolated from the rhizosphere of young M26 apple rootstocks, was investigated by a polyphasic taxonomic approach. Phylogenetic identification based on the full-length 16S rRNA gene sequence revealed highest 16S rRNA gene sequence similarity to the type strains of Rhodococcus wratislaviensis (99.6%) and Rhodococcus opacus (99.2%) followed by Rhodococcus imtechensis (98.9%). All other 16S rRNA gene sequence similarities were below 98.65%. A phylogenomic tree calculated based on a whole-genome sequence also showed a distinct clustering with the type strain of Rhodococcus koreensis. Average nucleotide identity (ANI) values between whole-genome sequences of R79 T and the closest related type strains were below 95% supported the novel species status. The DNA G + C content of R79 T was 67.24% mol. Predominant fatty acids were C 16:0, C 15:0 and C 17:1ω8c . The strain contained MK8-H 2 as the major respiratory quinone. The polar lipid profile consists of diphosphatidylglycerol and phosphatidylethanolamine, as well as of some unidentified lipids. The peptidoglycan type of the strain is A1γ meso-diaminopimelic acid. Based on the obtained genotypic and phenotypic, including chemotaxonomic data, we conclude that R79 T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus pseudokoreensis sp. nov. is proposed. The type strain is R79 T (= DSM 113102 T  = LMG 32444 T  = CCM 9183 T ).
(© 2022. The Author(s).)
Databáze: MEDLINE
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