Immunogenic properties of SARS-CoV-2 inactivated by ultraviolet light.

Autor: Gracheva AV; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia., Korchevaya ER; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia., Ammour YI; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia., Smirnova DI; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia., Sokolova OS; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia., Glukhov GS; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia., Moiseenko AV; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.; N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow, 119991, Russia., Zubarev IV; Faculty of Medicine, Lomonosov Moscow State University, Moscow, 119991, Russia., Samoilikov RV; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia., Leneva IA; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia., Svitich OA; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia.; F.F. Erisman Institute of Public Health, I.M. Sechenov First Moscow State Medical University, Moscow, 119991, Russia., Zverev VV; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia.; F.F. Erisman Institute of Public Health, I.M. Sechenov First Moscow State Medical University, Moscow, 119991, Russia., Faizuloev EB; I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia. faizuloev@mail.ru.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2022 Nov; Vol. 167 (11), pp. 2181-2191. Date of Electronic Publication: 2022 Jul 20.
DOI: 10.1007/s00705-022-05530-7
Abstrakt: Vaccination against COVID-19 is the most effective method of controlling the spread of SARS-CoV-2 and reducing mortality from this disease. The development of vaccines with high protective activity against a wide range of SARS-CoV-2 antigenic variants remains relevant. In this regard, evaluation of the effectiveness of physical methods of virus inactivation, such as ultraviolet irradiation (UV) of the virus stock, remains relevant. This study demonstrates that the UV treatment of SARS-CoV-2 completely inactivates its infectivity while preserving its morphology, antigenic properties, and ability to induce the production of virus-neutralizing antibodies in mice through immunization. Thus, the UV inactivation of SARS-CoV-2 makes it possible to obtain viral material similar in its antigenic and immunogenic properties to the native antigen, which can be used both for the development of diagnostic test systems and for the development of an inactivated vaccine against COVID-19.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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