Hair analysis for beta-blockers and calcium-channel blockers by using liquid chromatography-tandem mass spectrometry as a tool for monitoring adherence to antihypertensive therapy.

Autor: Taus F; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy., Gottardo R; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy., Ballotari M; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy., Utzeri C; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy., Tagliaro F; Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.; World-Class Research Center 'Digital biodesign and personalized healthcare', Sechenov First Moscow State Medical University, Moscow, Russia.
Jazyk: angličtina
Zdroj: Drug testing and analysis [Drug Test Anal] 2022 Oct; Vol. 14 (10), pp. 1773-1778. Date of Electronic Publication: 2022 Jul 21.
DOI: 10.1002/dta.3346
Abstrakt: Adherence to therapy is the key to a successful therapeutic intervention, especially in cardiovascular diseases in which a lack of adherence may have serious consequences in terms morbidity and/or mortality. In this context, hair analysis can be an excellent tool to monitor adherence to therapy. Indeed, drugs present in blood are incorporated into the hair matrix, where drugs and metabolites can stay unaltered for a long time protected from metabolism and degradation. In the present study, a simple, specific, and sensitive ultra-high performance liquid-chromatography-tandem mass spectrometry (UHPLC-MS/MS) method set up to determine in human hair seven beta-blockers (viz., metoprolol, sotalol, labetalol, atenolol, nebivolol, bisoprolol, and nadolol) and two calcium-channel blockers (lercanidipine and amlodipine), which are widely prescribed to treat medium-to-severe hypertensive conditions. The optimized method was successfully validated in terms of accuracy, repeatability, reproducibility, matrix effect and extraction recovery. Moreover, the applicability of the method was evaluated by analyzing 34 real samples of hair obtained from patients under long-term therapy with calcium channel blockers and beta-blockers.
(© 2022 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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