Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments.

Autor: Korolenya VA; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.; Novosibirsk National Research State University, Novosibirsk, Russia., Gavrilov KA; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.; Novosibirsk National Research State University, Novosibirsk, Russia., Sevost'ianova KS; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.; Novosibirsk National Research State University, Novosibirsk, Russia., Shevela AI; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.; Novosibirsk National Research State University, Novosibirsk, Russia., Filipenko ML; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.; Novosibirsk National Research State University, Novosibirsk, Russia., Smetanina MA; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. mariya_smetanina@niboch.nsc.ru.; Novosibirsk National Research State University, Novosibirsk, Russia. mariya_smetanina@niboch.nsc.ru.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 Jul; Vol. 173 (3), pp. 384-389. Date of Electronic Publication: 2022 Jul 19.
DOI: 10.1007/s10517-022-05554-8
Abstrakt: Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).
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Databáze: MEDLINE
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