Viral variant-resolved wastewater surveillance of SARS-CoV-2 at national scale.

Autor: Amman F; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.; Institute of Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Markt R; Department of Microbiology, Universität Innsbruck, Innsbruck, Austria., Endler L; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.; Institute of Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Hupfauf S; Department of Microbiology, Universität Innsbruck, Innsbruck, Austria., Agerer B; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria., Schedl A; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.; Institute of Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Richter L; Austrian Agency for Health and Food Safety (AGES), Vienna, Austria., Zechmeister M; dwh GmbH, Vienna, Austria., Bicher M; dwh GmbH, Vienna, Austria.; Institute for Information Systems Engineering, Technische Universität Wien, Vienna, Austria., Heiler G; Complexity Science Hub, Vienna, Austria.; Institute of Information Systems Engineering, Technische Universität Wien, Vienna, Austria., Triska P; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.; Institute of Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Thornton M; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.; Institute of Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Penz T; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria., Senekowitsch M; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria., Laine J; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria., Keszei Z; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria., Klimek P; Complexity Science Hub, Vienna, Austria.; Section for Science of Complex Systems, Medical University of Vienna, Vienna, Austria., Nägele F; Department of Microbiology, Universität Innsbruck, Innsbruck, Austria., Mayr M; Department of Microbiology, Universität Innsbruck, Innsbruck, Austria., Daleiden B; Institute of Legal Medicine and Core Facility Metabolomics, Medical University of Innsbruck, Innsbruck, Austria., Steinlechner M; Institute of Legal Medicine and Core Facility Metabolomics, Medical University of Innsbruck, Innsbruck, Austria., Niederstätter H; Institute of Legal Medicine and Core Facility Metabolomics, Medical University of Innsbruck, Innsbruck, Austria., Heidinger P; Austrian Centre of Industrial Biotechnology GmbH, Graz, Austria., Rauch W; Department of Infrastructure, Universität Innsbruck, Innsbruck, Austria., Scheffknecht C; Institut für Umwelt und Lebensmittelsicherheit des Landes Vorarlberg, Bregenz, Austria., Vogl G; Institut für Lebensmittelsicherheit, Veterinärmedizin und Umwelt des Landes Kärnten, Klagenfurt am Wörthersee, Austria., Weichlinger G; Abteilung 12 - Wasserwirtschaft, Amt der Kärntner Landesregierung, Klagenfurt am Wörthersee, Austria., Wagner AO; Department of Microbiology, Universität Innsbruck, Innsbruck, Austria., Slipko K; Institute for Water Quality and Resource Management, Technische Universität Wien, Vienna, Austria., Masseron A; Institute for Water Quality and Resource Management, Technische Universität Wien, Vienna, Austria., Radu E; Institute for Water Quality and Resource Management, Technische Universität Wien, Vienna, Austria.; Ştefan S. Nicolau Institute of Virology, Bucharest, Romania., Allerberger F; Austrian Agency for Health and Food Safety (AGES), Vienna, Austria., Popper N; dwh GmbH, Vienna, Austria.; Institute for Information Systems Engineering, Technische Universität Wien, Vienna, Austria., Bock C; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.; Institute of Artificial Intelligence, Center for Medical Statistics, Informatics, and Intelligent Systems, Medical University of Vienna, Vienna, Austria., Schmid D; Austrian Agency for Health and Food Safety (AGES), Vienna, Austria., Oberacher H; Institute of Legal Medicine and Core Facility Metabolomics, Medical University of Innsbruck, Innsbruck, Austria., Kreuzinger N; Institute for Water Quality and Resource Management, Technische Universität Wien, Vienna, Austria., Insam H; Department of Microbiology, Universität Innsbruck, Innsbruck, Austria., Bergthaler A; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria. andreas.bergthaler@meduniwien.ac.at.; Institute of Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria. andreas.bergthaler@meduniwien.ac.at.
Jazyk: angličtina
Zdroj: Nature biotechnology [Nat Biotechnol] 2022 Dec; Vol. 40 (12), pp. 1814-1822. Date of Electronic Publication: 2022 Jul 18.
DOI: 10.1038/s41587-022-01387-y
Abstrakt: SARS-CoV-2 surveillance by wastewater-based epidemiology is poised to provide a complementary approach to sequencing individual cases. However, robust quantification of variants and de novo detection of emerging variants remains challenging for existing strategies. We deep sequenced 3,413 wastewater samples representing 94 municipal catchments, covering >59% of the population of Austria, from December 2020 to February 2022. Our system of variant quantification in sewage pipeline designed for robustness (termed VaQuERo) enabled us to deduce the spatiotemporal abundance of predefined variants from complex wastewater samples. These results were validated against epidemiological records of >311,000 individual cases. Furthermore, we describe elevated viral genetic diversity during the Delta variant period, provide a framework to predict emerging variants and measure the reproductive advantage of variants of concern by calculating variant-specific reproduction numbers from wastewater. Together, this study demonstrates the power of national-scale WBE to support public health and promises particular value for countries without extensive individual monitoring.
(© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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