α-cyanobacteria possessing form IA RuBisCO globally dominate aquatic habitats.

Autor: Cabello-Yeves PJ; Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, San Juan de Alicante, Alicante, Spain. pedrito91vlc@gmail.com., Scanlan DJ; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Callieri C; National Research Council (CNR), Institute of Water Research (IRSA), Verbania, Italy., Picazo A; Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, E-46980 Paterna, Valencia, Spain., Schallenberg L; Department of Zoology, University of Otago, Dunedin, New Zealand., Huber P; Instituto Tecnológico de Chascomús (INTECH), UNSAM-CONICET., Av. Intendente Marino Km 8,200, 7130, Chascomús, Buenos Aires, Argentina.; Instituto Nacional de Limnología (INALI), CONICET-UNL., Ciudad Universitaria-Paraje el Pozo s/n, 3000, Santa Fé, Argentina., Roda-Garcia JJ; Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, San Juan de Alicante, Alicante, Spain., Bartosiewicz M; Department of Environmental Sciences, University of Basel, Basel, Switzerland., Belykh OI; Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033, Irkutsk, Russia., Tikhonova IV; Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033, Irkutsk, Russia., Torcello-Requena A; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., De Prado PM; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Millard AD; Department of Genetics and Genome Biology, University of Leicester, Leicester, LE1 7RH, UK., Camacho A; Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, E-46980 Paterna, Valencia, Spain., Rodriguez-Valera F; Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, San Juan de Alicante, Alicante, Spain.; Moscow Institute of Physics and Technology, 141701, Dolgoprudny, Russia., Puxty RJ; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK. r.puxty@warwick.ac.uk.
Jazyk: angličtina
Zdroj: The ISME journal [ISME J] 2022 Oct; Vol. 16 (10), pp. 2421-2432. Date of Electronic Publication: 2022 Jul 18.
DOI: 10.1038/s41396-022-01282-z
Abstrakt: RuBisCO (ribulose 1,5-bisphosphate carboxylase/oxygenase) is one the most abundant enzymes on Earth. Virtually all food webs depend on its activity to supply fixed carbon. In aerobic environments, RuBisCO struggles to distinguish efficiently between CO 2 and O 2 . To compensate, organisms have evolved convergent solutions to concentrate CO 2 around the active site. The genetic engineering of such inorganic carbon concentrating mechanisms (CCMs) into plants could help facilitate future global food security for humankind. In bacteria, the carboxysome represents one such CCM component, of which two independent forms exist: α and β. Cyanobacteria are important players in the planet's carbon cycle and the vast majority of the phylum possess a β-carboxysome, including most cyanobacteria used as laboratory models. The exceptions are the exclusively marine Prochlorococcus and Synechococcus that numerically dominate open ocean systems. However, the reason why marine systems favor an α-form is currently unknown. Here, we report the genomes of 58 cyanobacteria, closely related to marine Synechococcus that were isolated from freshwater lakes across the globe. We find all these isolates possess α-carboxysomes accompanied by a form 1A RuBisCO. Moreover, we demonstrate α-cyanobacteria dominate freshwater lakes worldwide. Hence, the paradigm of a separation in carboxysome type across the salinity divide does not hold true, and instead the α-form dominates all aquatic systems. We thus question the relevance of β-cyanobacteria as models for aquatic systems at large and pose a hypothesis for the reason for the success of the α-form in nature.
(© 2022. The Author(s).)
Databáze: MEDLINE
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