Macrophages Are Polarized toward an Inflammatory Phenotype by their Aged Microenvironment in the Human Skin.

Autor:	Gather L; Research & Development, Beiersdorf, Hamburg, Germany., Nath N; Institute of Bioinformatics, University Medicine Greifswald, Greifswald, Germany., Falckenhayn C; Research & Development, Beiersdorf, Hamburg, Germany., Oterino-Sogo S; Melanoma Laboratory, Spanish National Cancer Research Centre, Madrid, Spain., Bosch T; Zoological Institute, Kiel University, Kiel, Germany., Wenck H; Research & Development, Beiersdorf, Hamburg, Germany., Winnefeld M; Research & Development, Beiersdorf, Hamburg, Germany., Grönniger E; Research & Development, Beiersdorf, Hamburg, Germany., Simm S; Institute of Bioinformatics, University Medicine Greifswald, Greifswald, Germany., Siracusa A; Research & Development, Beiersdorf, Hamburg, Germany. Electronic address: Annette.Siracusa@beiersdorf.com.
Jazyk:	angličtina
Zdroj:	The Journal of investigative dermatology [J Invest Dermatol] 2022 Dec; Vol. 142 (12), pp. 3136-3145.e11. Date of Electronic Publication: 2022 Jul 16.
DOI:	10.1016/j.jid.2022.06.023
Abstrakt:	Aging of the skin is accompanied by cellular as well as tissue environmental changes, ultimately reducing the ability of the tissue to regenerate and adequately respond to external stressors. Macrophages are important gatekeepers of tissue homeostasis, and it has been reported that their number and phenotype change during aging in a site-specific manner. How aging affects human skin macrophages and what implications this has for the aging process in the tissue are still not fully understood. Using single-cell RNA-sequencing analysis, we show that there is at least a 50% increase of macrophages in human aged skin, which appear to have developed from monocytes and exhibit more proinflammatory M1-like characteristics. In contrast, the cell-intrinsic ability of aged monocytes to differentiate into M1 macrophages was reduced. Using coculture experiments with aged dermal fibroblasts, we show that it is the aged microenvironment that drives a more proinflammatory phenotype of macrophages in the skin. This proinflammatory M1-like phenotype in turn negatively influenced the expression of extracellular matrix proteins by fibroblasts, emphasizing the impact of the aged macrophages on the skin phenotype. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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