Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator.
| Autor: | Matlashov ME; Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States., Vera J; Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, United States., Kasatkina LA; Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States., Khodakhah K; Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, United States., Verkhusha VV; Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States.; Medicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in cell and developmental biology [Front Cell Dev Biol] 2022 Jun 29; Vol. 10, pp. 880107. Date of Electronic Publication: 2022 Jun 29 (Print Publication: 2022). |
| DOI: | 10.3389/fcell.2022.880107 |
| Abstrakt: | Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-scattering, and autofluorescence in mammalian tissues. In addition, NIR GECIs do not suffer from cross-excitation artifacts when used with common fluorescent indicators and optogenetics actuators. Although several NIR GECIs have been developed, there is no NIR GECI currently available that would combine the high brightness in cells and photostability with small size and fast response kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator iGECInano. We characterize iGECInano in vitro , in non-neuronal mammalian cells, and primary mouse neurons. iGECInano demonstrates the improvement in the signal-to-noise ratio and response kinetics compared to other NIR GECIs. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Matlashov, Vera, Kasatkina, Khodakhah and Verkhusha.) |
| Databáze: | MEDLINE |
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