Regulation and Role of Hypoxia-Induced Factor 1α (HIF-1α) under Conditions of Endogenous Oxidative Stress In Vitro.

Autor: Abalenikhina YV; Ryazan State Medical University, Ministry of Health of the Russian Federation, Ryazan, Russia. abalenihina88@mail.ru., Myl'nikov PY; Ryazan State Medical University, Ministry of Health of the Russian Federation, Ryazan, Russia., Shchul'kin AV; Ryazan State Medical University, Ministry of Health of the Russian Federation, Ryazan, Russia., Chernykh IV; Ryazan State Medical University, Ministry of Health of the Russian Federation, Ryazan, Russia., Yakusheva EN; Ryazan State Medical University, Ministry of Health of the Russian Federation, Ryazan, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 Jul; Vol. 173 (3), pp. 312-316. Date of Electronic Publication: 2022 Jul 18.
DOI: 10.1007/s10517-022-05540-0
Abstrakt: The effect of endogenous oxidative stress induced by γ-glutamyl cysteinesynthetase inhibitor D,L-buthionine sulfoximine (BSO) on the functioning of hypoxia-induced factor 1α (HIF-1α) was studied on Caco-2 cells. BSO was added for 24 h in concentrations of 5, 10, 50, 100, and 500 μM. It was shown that BSO in concentrations of 10, 50, and 100 μM induced endogenous oxidative stress and increased the content of HIF-1α; this effect was regulated through nuclear factor of erythroid origin 2 (Nrf2). Activation of HIF-1α had an independent protective effect, as evidenced by the decrease in cell viability after HIF-1α inhibition under these conditions. When the concentration of BSO was increased to 500 μM the content of HIF-1α did not change, and cell viability decreased.
(© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: