Toxocara canis extract fractions promote mainly the production of Th1 and regulatory cytokines by human leukocytes in vitro.

Autor: Amor DALM; Laboratório de Parasitologia, Centro de Ciências da Saúde, Universidade Federal do Recôncavo da Bahia, Avenida Carlos Amaral, 1015 - Cajueiro, Santo Antônio de Jesus, Bahia 44430-622, Brazil. Electronic address: ana_amor@ufrb.edu.br., Santos LN; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Silva ES; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., de Santana MBR; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Belitardo EMMA; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Sena FA; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Pontes-de-Carvalho L; Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil., Figueiredo CA; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Alcântara-Neves NM; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2022 Oct; Vol. 234, pp. 106579. Date of Electronic Publication: 2022 Jul 16.
DOI: 10.1016/j.actatropica.2022.106579
Abstrakt: Helminths possibly down-modulate immune responses to airborne allergens through the induction of a regulatory network. The identification of helminths bioactive molecules is highly desirable, given their immunomodulatory potential which could be used in immunotherapies for allergy and autoimmune diseases. To investigate the immunoregulatory potential of the adult Toxocara canis crude extract and ten protein fractions of its extract, human peripheral blood mononuclear cells (PBMC) from 10 allergic and 9 non-allergic individuals were cultivated, in vitro, in the presence or absence of these antigens, and their supernatants were evaluated for cytokine production (TGF-β, IL-10, IL-12, TNF-α, IL-6, IL-5, IL13, and IL-17). To determine the cell viability, the PBMC were cultivated for 24 h in the presence of the antigens and, following, they were subjected to a cytotoxicity assay. The viability of the PBMC was not affected by incubation with the T. canis antigens. As some fractions stimulated the production of immunoregulatory (TGF-β and/or IL-10), IL-12 and Th1 (TNF-α) cytokines, without stimulating Th2 cytokines (IL-5 and IL13) and IL-17, it was proposed that they would be potential candidates for further studies, especially involving the purification and characterization of specific proteins, which could be tested separately to evaluate their specific role as adjuvants in immunotherapy for inflammatory diseases.
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Databáze: MEDLINE
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