Hyaluronic acid-entecavir conjugates-core/lipid-shell nanohybrids for efficient macrophage uptake and hepatotropic prospects.

Autor: Hamdi M; Department of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, Egypt., Elmowafy E; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Egypt., Abdel-Bar HM; Department of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, Egypt; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, United Kingdom. Electronic address: hend.abdelbar@fop.usc.edu.eg., ElKashlan AM; Department of Biochemistry, Faculty of Pharmacy, University of Sadat City, Egypt., Al-Jamal KT; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, United Kingdom., Awad GAS; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Egypt.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2022 Sep 30; Vol. 217, pp. 731-747. Date of Electronic Publication: 2022 Jul 13.
DOI: 10.1016/j.ijbiomac.2022.07.067
Abstrakt: Drug covalently bound to polymers had formed, lately, platforms with great promise in drug delivery. These drug polymer conjugates (DPC) boosted drug loading and controlled medicine release with targeting ability. Herein, the ability of entecavir (E) conjugated to hyaluronic acid (HA) forming the core of vitamin E coated lipid nanohybrids (EE-HA LPH), to target Kupffer cells and hepatocyte had been proved. The drug was associated to HA with efficiency of 93.48 ± 3.14 % and nanohybrids loading of 22.02 ± 2.3 %. DiI labelled lipidic nanohybrids improved the macrophage uptake in J774 cells with a 21 day hepatocytes retention post intramuscular injection. Finally, in vivo biocompatibility and safety with respect to body weight, organs indices and histopathological alterations were demonstrated. Coating with vitamin E and conjugation of E to HA (a CD44 ligand), could give grounds for prospective application for vectored nano-platform in hepatitis B.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE