Screening of febrile patients with suspected malaria from the Brazilian Amazon for virus infection.

Autor: de Figueiredo MLG; Laboratory of Virology, Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Williams EP; Department of Microbiology, Immunology, and Biochemistry, The University of Tennessee Health Science Center, Memphis, TN, USA., Jonsson CB; Department of Microbiology, Immunology, and Biochemistry, The University of Tennessee Health Science Center, Memphis, TN, USA., Khan MJ; Laboratory of Virology, Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Nunes MRT; Evandro Chagas Institute, Rodovia BR-316, km 7 s/n, Levilândia, Ananindeua, Para, 67030-000, Brazil., de Lima CPS; Evandro Chagas Institute, Rodovia BR-316, km 7 s/n, Levilândia, Ananindeua, Para, 67030-000, Brazil., Figueiredo LTM; Ribeirao Preto Medical School, Virology Research Center, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Costa MRF; Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, Amazonas, Brazil., Mourão MPG; Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, Amazonas, Brazil.; Universidade do Estado do Amazonas (UEA), Manaus, Amazonas, Brazil., Lacerda MVG; Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, Amazonas, Brazil. marcuslacerda.br@gmail.com.; Instituto Leônidas and Maria Deane (FIOCRUZ-Amazonas), Fundação Oswaldo Cruz, Manaus, Amazonas, Brazil. marcuslacerda.br@gmail.com., Aquino VH; Laboratory of Virology, Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil. vhugo@fcfrp.usp.br.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2022 Nov; Vol. 167 (11), pp. 2151-2162. Date of Electronic Publication: 2022 Jul 16.
DOI: 10.1007/s00705-022-05514-7
Abstrakt: Arthropod-borne viruses (arboviruses) are a significant public health threat, especially in tropical and subtropical regions. More than 150 arboviruses can cause febrile illness following infection in humans. The Brazilian Amazon region has the highest number of arboviruses detected worldwide. In addition to arboviruses, malaria, caused by Plasmodium vivax, is endemic in the Amazon. Patients with malaria and arboviral disease frequently show similar clinical presentation and laboratory findings, making the diagnosis of the cause of the infection challenging. The aim of this study was to evaluate the potential for viral infections in patients with suspected malaria but without Plasmodium infection in the Brazilian Amazon. We recruited 200 subjects with suspected malaria in Manaus, Brazil. First, we tested for arboviruses in serum samples from 124 of the 200 participants using an arbovirus DNA microarray platform, which did not detect any virus. Then, we mixed the serum samples of the other 76 participants in 10 pools and subjected them to next-generation sequencing. Analysis of the sequencing data revealed the presence of only one arbovirus (Zika virus) in one sample pool. This analysis also detected the presence of primate erythroparvovirus 1 and pegivirus C. These results suggest that arboviruses are not the most frequent viral infections in patients with suspected malaria but without Plasmodium infection in the metropolitan region of Manaus. Implementation of specific viral surveillance tests will help in the early detection of viruses with epidemic potential.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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