Significance of chlorine-dioxide-based oral rinses in preventing SARS-CoV-2 cell entry.

Autor: Travis BJ; College of Dental Medicine, Midwestern University, Downers Grove, Illinois, USA., Elste J; Department of Microbiology and Immunology, Midwestern University, Downers Grove, Illinois, USA., Gao F; College of Dental Medicine, Midwestern University, Downers Grove, Illinois, USA., Joo BY; Department of Microbiology and Immunology, Midwestern University, Downers Grove, Illinois, USA., Cuevas-Nunez M; College of Dental Medicine, Midwestern University, Downers Grove, Illinois, USA., Kohlmeir E; Core Facility, Midwestern University, Illinois, Downers Grove, Illinois, USA., Tiwari V; Department of Microbiology and Immunology, Midwestern University, Downers Grove, Illinois, USA., Mitchell JC; College of Dental Medicine, Midwestern University, Downers Grove, Illinois, USA.
Jazyk: angličtina
Zdroj: Oral diseases [Oral Dis] 2022 Nov; Vol. 28 Suppl 2, pp. 2481-2491. Date of Electronic Publication: 2022 Jul 28.
DOI: 10.1111/odi.14319
Abstrakt: Objective: This work aims to determine the efficacy of preprocedural oral rinsing with chlorine dioxide solutions to minimize the risk of coronavirus disease 2019 (COVID-19) transmission during high-risk dental procedures.
Methods: The antiviral activity of chlorine-dioxide-based oral rinse (OR) solutions was tested by pre-incubating with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus in a dosage-dependent manner before transducing to human embryonic kidney epithelial (HEK293T-ACE2) cells, which stably expresses ACE-2 receptor. Viral entry was determined by measuring luciferase activity using a luminescence microplate reader. In the cell-to-cell fusion assay, effector Chinese hamster ovary (CHO-K1) cells co-expressing spike glycoprotein of SARS-CoV-2 and T7 RNA polymerase were pre-incubated with the ORs before co-culturing with the target CHO-K1 cells co-expressing human ACE2 receptor and luciferase gene. The luciferase signal was quantified 24 h after mixing the cells. Surface expression of SARS-CoV-2 spike glycoprotein and ACE-2 receptor was confirmed using direct fluorescent imaging and quantitative cell-ELISA. Finally, dosage-dependent cytotoxic effects of ORs were evaluated at two different time points.
Results: A dosage-dependent antiviral effect of the ORs was observed against SARS-CoV-2 cell entry and spike glycoprotein mediated cell-to-cell fusion. This demonstrates that ORs can be useful as a preprocedural step to reduce viral infectivity.
Conclusions: Chlorine-dioxide-based ORs have a potential benefit for reducing SARS-CoV-2 entry and spread.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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