Evaluation Protocol for SARS-CoV-2 Serological Assays.

Autor:	Gededzha MP; Department of Immunology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Maemu.gededzha@nhls.ac.za.; National Health Laboratory Services, Johannesburg, South Africa. Maemu.gededzha@nhls.ac.za., Jugwanth S; Department of Immunology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.; National Health Laboratory Services, Johannesburg, South Africa., Mampeule N; Department of Immunology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.; National Health Laboratory Services, Johannesburg, South Africa., Zwane N; Department of Immunology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.; National Health Laboratory Services, Johannesburg, South Africa., David A; Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, South Africa., Scott L; National Health Laboratory Services, Johannesburg, South Africa.; Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, South Africa., Stevens W; National Health Laboratory Services, Johannesburg, South Africa.; Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, South Africa., Mayne ES; Department of Immunology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.; National Health Laboratory Services, Johannesburg, South Africa.; Division of Immunology, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2511, pp. 307-319.
DOI:	10.1007/978-1-0716-2395-4_23
Abstrakt:	Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of COVID-19. Accurate detection of SARS-CoV-2 infection is not only important for management of infected individuals but also to break the chain of transmission. Although the polymerase chain reaction (PCR) is the gold standard for diagnosis of acute SARS-CoV-2 infection, there are a number of limitations of these assays, which include the inability to detect past infection and decline in sensitivity 14 days post-symptom onset. There are several serology tests developed for the detection of SARS-CoV-2 antibodies including high-throughput serology platforms and lateral flow immunoassays. These tests should be evaluated for their performance to meet local regulations acceptance criteria. To optimize the diagnostic algorithm for SARS-CoV-2, this protocol describes the evaluation of serological antibody testing using various automated serology platforms and lateral flow immunoassays. This protocol was evaluated in both serum and plasma samples. The sample preparation, procedure, and data analysis are described. The protocol can be adapted for any serological testing. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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