3D culturing of human pluripotent stem cells-derived endothelial cells for vascular regeneration.

Autor:	Gara E; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Zucchelli E; National Heart and Lung Institute, Imperial College London, W12 0NN, United Kingdom., Nemes A; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Jakus Z; Department of Physiology, Semmelweis University, Budapest, H1094, Hungary.; MTA-SE 'Lendület' Lymphatic Physiology Research Group of the Hungarian Academy of Sciences and the Semmelweis University, Budapest, H1094, Hungary., Ajtay K; Department of Physiology, Semmelweis University, Budapest, H1094, Hungary.; MTA-SE 'Lendület' Lymphatic Physiology Research Group of the Hungarian Academy of Sciences and the Semmelweis University, Budapest, H1094, Hungary., Kemecsei É; Department of Physiology, Semmelweis University, Budapest, H1094, Hungary.; MTA-SE 'Lendület' Lymphatic Physiology Research Group of the Hungarian Academy of Sciences and the Semmelweis University, Budapest, H1094, Hungary., Kiszler G; 3D HISTECH Ltd., Budapest, H1141, Hungary., Hegedűs N; Department of Biophysics and Radiation Biology, Nanobiotechnology & In vivo Imaging Center, Semmelweis University, H1094, Budapest, Hungary and In vivo Imaging Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine. www.hcemm.eu, Szeged, Hungary., Szigeti K; Department of Biophysics and Radiation Biology, Nanobiotechnology & In vivo Imaging Center, Semmelweis University, H1094, Budapest, Hungary and In vivo Imaging Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine. www.hcemm.eu, Szeged, Hungary., Földes I; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Árvai K; Department of Internal Medicine and Oncology, Semmelweis University; PentaCore Laboratory, Budapest, H1083, Hungary., Kósa J; Department of Internal Medicine and Oncology, Semmelweis University; PentaCore Laboratory, Budapest, H1083, Hungary., Kolev K; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, H1094, Hungary., Komorowicz E; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, H1094, Hungary., Padmanabhan P; Lee Kong Chian School of Medicine, Imperial College - Nanyang Technological University, 636921, Singapore., Maurovich-Horvat P; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Dósa E; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Várady G; Research Centre for Natural Sciences, Budapest, H1117, Hungary., Pólos M; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Hartyánszky I; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Harding SE; National Heart and Lung Institute, Imperial College London, W12 0NN, United Kingdom., Merkely B; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Máthé D; Department of Biophysics and Radiation Biology, Nanobiotechnology & In vivo Imaging Center, Semmelweis University, H1094, Budapest, Hungary and In vivo Imaging Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine. www.hcemm.eu, Szeged, Hungary., Szabó G; Experimentelle Herzchirurgie, Ruprecht-Karls Universität, Heidelberg, 69120, Germany.; Department of Cardiac Surgery, University of Halle, Halle (Saale), 06108, Germany., Radovits T; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary., Földes G; Heart and Vascular Center, Semmelweis University, Budapest, H1122, Hungary.; National Heart and Lung Institute, Imperial College London, W12 0NN, United Kingdom.
Jazyk:	angličtina
Zdroj:	Theranostics [Theranostics] 2022 Jun 06; Vol. 12 (10), pp. 4684-4702. Date of Electronic Publication: 2022 Jun 06 (Print Publication: 2022).
DOI:	10.7150/thno.69938
Abstrakt:	Rationale: Human induced pluripotent stem cell-derived endothelial cells can be candidates for engineering therapeutic vascular grafts. Methods: Here, we studied the role of three-dimensional culture on their characteristics and function both in vitro and in vivo . Results: We found that differentiated hPSC-EC can re-populate decellularized biomatrices; they remain viable, undergo maturation and arterial/venous specification. Human PSC-EC develop antifibrotic, vasoactive and anti-inflammatory properties during recellularization. In vivo , a robust increase in perfusion was detected at the engraftment sites after subcutaneous implantation of an hPSC-EC-laden hydrogel in rats. Histology confirmed survival and formation of capillary-like structures, suggesting the incorporation of hPSC-EC into host microvasculature. In a canine model, hiPSC-EC-seeded onto decellularised vascular segments were functional as aortic grafts. Similarly, we showed the retention and maturation of hiPSC-EC and dynamic remodelling of the vessel wall with good maintenance of vascular patency. Conclusions: A combination of hPSC-EC and biomatrices may be a promising approach to repair ischemic tissues. Competing Interests: Competing Interests: DM is managing director, CROmed Research Ltd, grant-in-aid of Mediso Ltd. by providing nanoScan PET-MRI instrument usage time. (© The author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu