Aging Leads to Increased Monocytes and Macrophages With Altered CSF-1 Receptor Expression and Earlier Tumor-Associated Macrophage Expansion in Murine Mesothelioma.

Autor:	Duong L; Curtin Medical School, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, WA, Australia., Pixley FJ; School of Biomedical Sciences, The University of Western Australia, Perth, WA, Australia., Nelson DJ; Curtin Medical School, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, WA, Australia., Jackaman C; Curtin Medical School, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, WA, Australia.
Jazyk:	angličtina
Zdroj:	Frontiers in aging [Front Aging] 2022 Apr 27; Vol. 3, pp. 848925. Date of Electronic Publication: 2022 Apr 27 (Print Publication: 2022).
DOI:	10.3389/fragi.2022.848925
Abstrakt:	Increased cancer incidence occurs with the emergence of immunosenescence, highlighting the indispensability of the immune system in preventing cancer and its dysregulation with aging. Tumor-associated macrophages (TAMs) are often present in high numbers and are associated with poor clinical outcomes in solid cancers, including mesothelioma. Monocytes and macrophages from the bone marrow and spleen can respond to tumor-derived factors, such as CSF-1, and initiation of the CSF-1R signaling cascade results in their proliferation, differentiation, and migration to the tumor. Age-related changes occur in monocytes and macrophages in terms of numbers and function, which in turn can impact tumor initiation and progression. Whether this is due to changes in CSF-1R expression with aging is currently unknown and was investigated in this study. We examined monocytes and macrophages in the bone marrow and spleen during healthy aging in young (3-4 months) and elderly (20-24 months) female C57BL/6J mice. Additionally, changes to these tissues and in TAMs were examined during AE17 mesothelioma tumor growth. Healthy aging resulted in an expansion of Ly6C high monocytes and macrophages in the bone marrow and spleen. CSF-1R expression levels were reduced in elderly splenic macrophages only, suggesting differences in CSF-1R signaling between both cell type and tissue site. In tumor-bearing mice, Ly6C high monocytes increased with tumor growth in the spleen in the elderly and increased intracellular CSF-1R expression occurred in bone marrow Ly6C high monocytes in elderly mice bearing large tumors. Age-related changes to bone marrow and splenic Ly6C high monocytes were reflected in the tumor, where we observed increased Ly6C high TAMs earlier and expansion of Ly6C low TAMs later during AE17 tumor growth in the elderly compared to young mice. F4/80 high TAMs increased with tumor growth in both young and elderly mice and were the largest subset of TAMs in the tumor. Together, this suggests there may be a faster transition of Ly6C high towards F4/80 high TAMs with aging. Amongst TAM subsets, expression of CSF-1R was lowest in F4/80 high TAMs, however Ly6C low TAMs had higher intracellular CSF-1R expression. This suggests downstream CSF-1R signaling may vary between macrophage subsets, which can have implications towards CSF-1R blockade therapies targeting macrophages in cancer. Competing Interests: DN acts as a non-salaried Chief Scientific Officer for Selvax. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Duong, Pixley, Nelson and Jackaman.)
Databáze:	MEDLINE
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