Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-relapse Compounds.

Autor:	Voorberg-van der Wel AM; Department of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands., Zeeman AM; Department of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands., Nieuwenhuis IG; Department of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands., van der Werff NM; Department of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands., Kocken CHM; Department of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands. kocken@bprc.nl.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2524, pp. 397-408.
DOI:	10.1007/978-1-0716-2453-1_31
Abstrakt:	Malaria hypnozoites are dormant parasite stages that reside inside hepatocytes. Upon activation, these stages can resume growth, causing new episodes of blood stage malaria infection. This chapter describes a fast and sensitive protocol for the detection of bioluminescent (BL) hypnozoites in vitro. Using transgenic Plasmodium cynomolgi parasites that differentially express the BL reporter proteins firefly luciferase and the ultrabright NanoLuc, hypnozoites can be distinguished from liver stage schizonts. This robust method sets the stage for implementation in large-scale drug screening platforms with the aim to find new compounds that eliminate hypnozoites. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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