Genetic characterization of Toxoplasma gondii in meat-producing animals in Iran.

Autor: Amouei A; Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.; Department of Parasitology, School of Medicine, Mazandaran University of Medical Science, Sari, 4847191971, Mazandaran, Iran., Sarvi S; Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.; Department of Parasitology, School of Medicine, Mazandaran University of Medical Science, Sari, 4847191971, Mazandaran, Iran., Mizani A; Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran., Hashemi-Soteh MB; Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran., Salehi S; Mazandaran Provincial Veterinary Department of Medical Sciences, Sari, Iran., Javidnia J; Department of Mycology, School of Medicine, Mazandaran University of Medical Science, Sari, Iran., Hosseini SA; Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.; Department of Parasitology, School of Medicine, Mazandaran University of Medical Science, Sari, 4847191971, Mazandaran, Iran., Amuei F; Department of Organic Chemistry, University of Mazandaran, Babolsar, Iran., Alizadeh A; Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran., Shabanzade S; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran., Gholami S; Department of Parasitology, School of Medicine, Mazandaran University of Medical Science, Sari, 4847191971, Mazandaran, Iran., Daryani A; Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran. daryanii@yahoo.com.; Department of Parasitology, School of Medicine, Mazandaran University of Medical Science, Sari, 4847191971, Mazandaran, Iran. daryanii@yahoo.com.
Jazyk: angličtina
Zdroj: Parasites & vectors [Parasit Vectors] 2022 Jul 11; Vol. 15 (1), pp. 255. Date of Electronic Publication: 2022 Jul 11.
DOI: 10.1186/s13071-022-05360-1
Abstrakt: Background: The consumption of uncooked or undercooked food from infected intermediate hosts can result in Toxoplasma gondii infection in humans. However, few studies have investigated the genetic diversity of this protozoan parasite in Iran. The aim of the present study was to genetically characterize isolates of T. gondii from intermediate host animals in Mazandaran Province, Iran.
Methods: Blood and heart tissue samples were collected from 204 ruminants, and brain tissue was collected from 335 birds. The prevalence of T. gondii infection in these samples was determined serologically using the modified agglutination test and by conventional PCR assays. Those PCR samples positive for T. gondii DNA and 13 DNA samples extracted from aborted fetuses in a previous study were genotyped with 12 genetic markers using the multilocus-nested PCR-restriction fragment length polymorphism (Mn-PCR-RFLP) technique.
Results: Antibodies for parasites were found in 35.7% of the ruminant (39.1% of sheep and 26.4% of goats) samples and in 51.3% of the bird (100% of geese, 52.3% of free-range chickens and 46% of ducks) samples. Molecular detection by PCR of the repetitive 529-bp DNA fragment revealed contamination of 13.2% of ruminant (14.6% of sheep and 9.4% of goats) samples and of 9.6% of bird (11.1% of chickens, 5.7% of ducks and 0% of geese samples). The results from 30 DNA samples revealed five ToxoDB (genome database for the genus Toxoplasma) PCR-RFLP genotypes, including #1 (Type II), #2 (Type III), #10 (Type I), #27 and #48, with genotype #1 the most predominant.
Conclusions: As evidenced by the results of this study, ruminants and birds are infected with T. gondii in the region, suggesting that they are likely to be involved in the transmission of T. gondii to humans through meat consumption. The identification of different genotypes may suggest a higher genetic diversity of this parasite in Mazandaran, reflecting local environmental contamination. These results have important public health implications for the prevention and control strategies of infection.
(© 2022. The Author(s).)
Databáze: MEDLINE
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