Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes.

Autor:	Piffoux M; Laboratoire Matières et Systèmes Complexes, CNRS UMR 7057, Université de Paris, Paris, France.; Interaction Cellules Souches-Niches: Physiologie, Tumeurs Et Réparation Tissulaire, Inserm UMR 1197, Université Paris-Saclay, Villejuif, France.; Département d'oncologie Médicale, Centre Léon Bérard, Lyon, France.; Oncology Department, CITOHL, Lyon-Sud Hospital, Cancer Institute of Hospices Civils de Lyon (IC-HCL), Hospices Civils de Lyon, Lyon, France., Silva AKA; Laboratoire Matières et Systèmes Complexes, CNRS UMR 7057, Université de Paris, Paris, France., Gazeau F; Laboratoire Matières et Systèmes Complexes, CNRS UMR 7057, Université de Paris, Paris, France., Tareste D; Institut de Psychiatrie et Neurosciences de Paris, Inserm UMR 1266, Université de Paris, Paris, France. david.tareste@inserm.fr.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2473, pp. 385-396.
DOI:	10.1007/978-1-0716-2209-4_24
Abstrakt:	Extracellular vesicles (EVs) and liposomes are natural and synthetic drug delivery systems, respectively, with their own advantages and limitations. EV/liposome fusion allows the generation of hybrid EVs that benefit from both the versatility of liposomes (tunable lipid and protein composition, surface functionalization, lumen loading, etc.) and the functionality of EVs (natural targeting properties, low immunogenicity, anti-inflammatory properties, etc.). Here, we describe the methods to (1) produce EVs and liposomes, (2) induce and monitor their fusion, and (3) purify the obtained hybrid EVs. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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