Boswellic acids, as novel inhibitor targeting peptidoglycan biosynthetic enzyme UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) in Escherichia coli.

Autor: Raina D; Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India.; Fermentation Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India.; Academy of Scientific and Innovative Research, (AcSIR), Ghaziabad, 201002, India., Khan FG; Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India., Tiwari H; Discovery Informatics, NPMC Division, CSIR-Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, 180001, India.; Academy of Scientific and Innovative Research, (AcSIR), Ghaziabad, 201002, India., Sangwan PL; Natural Products and Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India., Nargotra A; Discovery Informatics, NPMC Division, CSIR-Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu, 180001, India.; Academy of Scientific and Innovative Research, (AcSIR), Ghaziabad, 201002, India., Kumar V; Fermentation Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India.; Academy of Scientific and Innovative Research, (AcSIR), Ghaziabad, 201002, India., Khan IA; Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India. inshad@rediffmail.com.; Department of Microbiology, School of Life Sciences, Central University of Rajasthan, Ajmer, Rajasthan, 305817, India. inshad@rediffmail.com., Saran S; Fermentation Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, 180001, India. ssaran@iiim.res.in.; Academy of Scientific and Innovative Research, (AcSIR), Ghaziabad, 201002, India. ssaran@iiim.res.in.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2022 Jul 12; Vol. 204 (8), pp. 472. Date of Electronic Publication: 2022 Jul 12.
DOI: 10.1007/s00203-022-03066-7
Abstrakt: UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) is an essential cytosolic enzyme in the biosynthesis of peptidoglycan. It becomes a potential bacterial target for screening promising antibacterial compounds as it is associated with the early phases of peptidoglycan production. MurA enzyme is conserved and necessary for bacterial viability with no mammalian homolog, which is a well-proven therapeutic research target. The present study reports the natural compounds from Boswellia serrata targeting the MurA enzyme. The identified inhibitors against MurA Escherichia coli (E. coli): β-boswellic acid (IC 50 33.65 µM), Acetyl-β-boswellic acid (IC 50 30.17 µM), and Acetyl-11-keto-β-boswellic acid (IC 50 37.67 µM). Inhibitors showed a fourfold decrease in IC 50 values on pre-incubation with substrate-UDP-N-acetyl-glucosamine (UDP-GlcNAc). Mode-of-inhibition studies revealed their uncompetitive nature with both the substrates. Although these boswellic acids have been explored for their pharmacological potential, this is the first study reporting these compounds' E. coli MurA inhibiting potential.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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