Protective effect of green synthesized Selenium Nanoparticles against Doxorubicin induced multiple adverse effects in Swiss albino mice.

Autor: Khan MA; Molecular Cancer Genetics & Translational Research Lab, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India., Singh D; Molecular Cancer Genetics & Translational Research Lab, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India., Arif A; Department of Biochemistry, Aligarh Muslim University, Aligarh 202002, India., Sodhi KK; Soil Microbial Ecology and Environmental Toxicology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India; Hansraj College, University of Delhi, Delhi 110007, India., Singh DK; Hansraj College, University of Delhi, Delhi 110007, India., Islam SN; Interdisciplinary Nanotechnology Centre, Aligarh Muslim University, Aligarh 202002, India., Ahmad A; Interdisciplinary Nanotechnology Centre, Aligarh Muslim University, Aligarh 202002, India., Akhtar K; Department of Pathology, JNMC, Aligarh 202002, India., Siddique HR; Molecular Cancer Genetics & Translational Research Lab, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India. Electronic address: hifzur.zo@amu.ac.in.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2022 Sep 15; Vol. 305, pp. 120792. Date of Electronic Publication: 2022 Jul 09.
DOI: 10.1016/j.lfs.2022.120792
Abstrakt: Aims: Doxorubicin (DOX) is a widely used drug against multiple cancers. However, its clinical Use is often restricted due to multiple adverse effects. Recently, Selenium Nanoparticles (SeNPs) are gaining attention due to their low toxicity and higher biocompatibility, making them attractive nanoparticles (NPs) in medical and pharmaceutical sciences. Therefore, the current study aimed to assess if our biosynthesized SeNP from the endophytic fungus Fusarium oxysporum conjugated with DOX could alleviate the DOX-induced adverse effects.
Main Methods: For this purpose, we investigated various genotoxic, biochemical, histopathological, and immunohistochemical parameters and finally analyzed the metabolite profile by LC-MS/MS.
Key Findings: We observed that DOX causes an increase in reactive oxygen and nitrogen species (ROS, RNS), 8-OHdG, and malondialdehyde (MDA), decreases antioxidant defense systems and reduces BCL-2 expression in cardiac tissue. In addition, a significant increase in DNA damage and alteration in the cytoarchitecture of the liver, kidney, and heart tissues was observed by Comet Tail Length and histopathological studies, respectively. Interestingly, the DOX-SeNP conjugate reduced ROS/RNS, 8-OHdG, and MDA levels in the liver, kidney, and heart tissues. It also restored the antioxidant enzymes and cytoarchitectures of the examined tissues, reduced genotoxicity, and increased the BCL-2 levels. Finally, metabolic profiling showed that DOX reduced the number of cardioprotective metabolites, which DOX-SeNP restored.
Significance: Collectively, the present results describe the protective effect of DOX-conjugated SeNP against DOX-induced toxicities. In conclusion, DOX-SeNP conjugate might be better for treating patients receiving DOX alone. However, it warrants further thorough investigation.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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