A Phytophthora effector promotes homodimerization of host transcription factor StKNOX3 to enhance susceptibility.

Autor:	Zhou J; Key Laboratory of Horticultural Plant Biology (HZAU), Ministry of Education, Huazhong Agricultural University (HZAU), Wuhan, Hubei, China.; Key Laboratory of Potato Biology and Biotechnology (HZAU), Ministry of Agriculture and Rural Affairs, Wuhan, China.; Potato Engineering and Technology Research Center of Hubei Province (HZAU), Wuhan, China.; Hubei Hongshan Laboratory (HZAU), Hubei Province, Wuhan, China., Qi Y; Key Laboratory of Horticultural Plant Biology (HZAU), Ministry of Education, Huazhong Agricultural University (HZAU), Wuhan, Hubei, China.; Key Laboratory of Potato Biology and Biotechnology (HZAU), Ministry of Agriculture and Rural Affairs, Wuhan, China.; Potato Engineering and Technology Research Center of Hubei Province (HZAU), Wuhan, China., Nie J; Key Laboratory of Horticultural Plant Biology (HZAU), Ministry of Education, Huazhong Agricultural University (HZAU), Wuhan, Hubei, China.; Key Laboratory of Potato Biology and Biotechnology (HZAU), Ministry of Agriculture and Rural Affairs, Wuhan, China.; Potato Engineering and Technology Research Center of Hubei Province (HZAU), Wuhan, China., Guo L; College of Agronomy, Northeast Agricultural University, Harbin, China., Luo M; Key Laboratory of Horticultural Plant Biology (HZAU), Ministry of Education, Huazhong Agricultural University (HZAU), Wuhan, Hubei, China.; Key Laboratory of Potato Biology and Biotechnology (HZAU), Ministry of Agriculture and Rural Affairs, Wuhan, China.; Potato Engineering and Technology Research Center of Hubei Province (HZAU), Wuhan, China., McLellan H; Division of Plant Sciences, University of Dundee, At James Hutton Institute, Invergowrie, Dundee, UK., Boevink PC; Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee, UK., Birch PRJ; Division of Plant Sciences, University of Dundee, At James Hutton Institute, Invergowrie, Dundee, UK.; Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee, UK., Tian Z; Key Laboratory of Horticultural Plant Biology (HZAU), Ministry of Education, Huazhong Agricultural University (HZAU), Wuhan, Hubei, China.; Key Laboratory of Potato Biology and Biotechnology (HZAU), Ministry of Agriculture and Rural Affairs, Wuhan, China.; Potato Engineering and Technology Research Center of Hubei Province (HZAU), Wuhan, China.; Hubei Hongshan Laboratory (HZAU), Hubei Province, Wuhan, China.
Jazyk:	angličtina
Zdroj:	Journal of experimental botany [J Exp Bot] 2022 Nov 02; Vol. 73 (19), pp. 6902-6915.
DOI:	10.1093/jxb/erac308
Abstrakt:	Oomycete pathogens secrete hundreds of cytoplasmic RxLR effectors to modulate host immunity by targeting diverse plant proteins. Revealing how effectors manipulate host proteins is pivotal to understanding infection processes and to developing new strategies to control plant disease. Here we show that the Phytophthora infestans RxLR effector Pi22798 interacts in the nucleus with a potato class II knotted-like homeobox (KNOX) transcription factor, StKNOX3. Silencing the ortholog NbKNOX3 in Nicotiana benthamiana reduces host colonization by P. infestans, whereas transient and stable overexpression of StKNOX3 enhances infection. StKNOX3 forms a homodimer which is dependent on its KNOX II domain. The KNOX II domain is also essential for Pi22798 interaction and for StKNOX3 to enhance P. infestans colonization, indicating that StKNOX3 homodimerization contributes to susceptibility. However, critically, the effector Pi22798 promotes StKNOX3 homodimerization, rather than heterodimerization to another KNOX transcription factor StKNOX7. These results demonstrate that the oomycete effector Pi22798 increases pathogenicity by promoting homodimerization specifically of StKNOX3 to enhance susceptibility. (© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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