Plasmodium knowlesi Cytoadhesion Involves SICA Variant Proteins.

Autor:	Peterson MS; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., Joyner CJ; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States.; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, United States., Lapp SA; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., Brady JA; School of Chemical, Materials and Biomedical Engineering, University of Georgia, Athens, GA, United States., Wood JS; Division of Animal Resources, Yerkes National Primate Research Center, Emory University, Atlanta, GA, United States., Cabrera-Mora M; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., Saney CL; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., Fonseca LL; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, United States., Cheng WT; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, United States., Jiang J; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., Soderberg SR; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., Nural MV; Institute of Bioinformatics, University of Georgia, Athens, GA, United States., Hankus A; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., Machiah D; Division of Pathology, Yerkes National Primate Research Center, Atlanta, GA, United States., Karpuzoglu E; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., DeBarry JD; Institute of Bioinformatics, University of Georgia, Athens, GA, United States., Tirouvanziam R; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, United States., Kissinger JC; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, United States.; Institute of Bioinformatics, University of Georgia, Athens, GA, United States.; Department of Genetics, University of Georgia, Athens, GA, United States., Moreno A; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States.; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, United States., Gumber S; Division of Pathology, Yerkes National Primate Research Center, Atlanta, GA, United States.; Department of Pathology and Laboratory Medicine, Emory School of Medicine, Atlanta, GA, United States., Voit EO; The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, United States., Gutierrez JB; Department of Mathematics, University of Georgia, Athens, GA, United States., Cordy RJ; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States., Galinski MR; Emory National Primate Research Center, Emory University, Atlanta, GA, United States.; Emory Vaccine Center, Emory University, Atlanta, GA, United States.; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Jun 23; Vol. 12, pp. 888496. Date of Electronic Publication: 2022 Jun 23 (Print Publication: 2022).
DOI:	10.3389/fcimb.2022.888496
Abstrakt:	Plasmodium knowlesi poses a health threat throughout Southeast Asian communities and currently causes most cases of malaria in Malaysia. This zoonotic parasite species has been studied in Macaca mulatta (rhesus monkeys) as a model for severe malarial infections, chronicity, and antigenic variation. The phenomenon of Plasmodium antigenic variation was first recognized during rhesus monkey infections. Plasmodium -encoded variant proteins were first discovered in this species and found to be expressed at the surface of infected erythrocytes, and then named the Schizont-Infected Cell Agglutination (SICA) antigens. SICA expression was shown to be spleen dependent, as SICA expression is lost after P. knowlesi is passaged in splenectomized rhesus. Here we present data from longitudinal P. knowlesi infections in rhesus with the most comprehensive analysis to date of clinical parameters and infected red blood cell sequestration in the vasculature of tissues from 22 organs. Based on the histopathological analysis of 22 tissue types from 11 rhesus monkeys, we show a comparative distribution of parasitized erythrocytes and the degree of margination of the infected erythrocytes with the endothelium. Interestingly, there was a significantly higher burden of parasites in the gastrointestinal tissues, and extensive margination of the parasites along the endothelium, which may help explain gastrointestinal symptoms frequently reported by patients with P. knowlesi malarial infections. Moreover, this margination was not observed in splenectomized rhesus that were infected with parasites not expressing the SICA proteins. This work provides data that directly supports the view that a subpopulation of P. knowlesi parasites cytoadheres and sequesters, likely via SICA variant antigens acting as ligands. This process is akin to the cytoadhesive function of the related variant antigen proteins, namely Erythrocyte Membrane Protein-1, expressed by Plasmodium falciparum . Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Peterson, Joyner, Lapp, Brady, Wood, Cabrera-Mora, Saney, Fonseca, Cheng, Jiang, Soderberg, Nural, Hankus, Machiah, Karpuzoglu, DeBarry, MaHPIC-Consortium, Tirouvanziam, Kissinger, Moreno, Gumber, Voit, Gutierrez, Cordy and Galinski.)
Databáze:	MEDLINE
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