| Autor: |
Kulikouskaya VI; Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 Skariny Str., 220141 Minsk, Belarus., Nikalaichuk VV; Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 Skariny Str., 220141 Minsk, Belarus., Bonartsev AP; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia., Akoulina EA; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia.; Biological Faculty, Shenzhen MSU-BIT University, Ruyi Rd. 299, Longgang District, Shenzhen 518172, China., Belishev NV; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia., Demianova IV; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia.; Biological Faculty, Shenzhen MSU-BIT University, Ruyi Rd. 299, Longgang District, Shenzhen 518172, China., Chesnokova DV; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia., Makhina TK; Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Science, Leninsky Prospect, 33, Build. 2, 119071 Moscow, Russia., Bonartseva GA; Federal Research Centre 'Fundamentals of Biotechnology' of the Russian Academy of Science, Leninsky Prospect, 33, Build. 2, 119071 Moscow, Russia., Shaitan KV; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia., Hileuskaya KS; Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 Skariny Str., 220141 Minsk, Belarus., Voinova VV; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia. |
| Abstrakt: |
Surface morphology affects cell attachment and proliferation. In this research, different films made of biodegradable polymers, poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (PHB- co -HV), containing different molecular weights, with microstructured surfaces were investigated. Two methods were used to obtain patterned films-water-assisted self-assembly ("breath figure") and spin-coating techniques. The water-assisted technique made it possible to obtain porous films with a self-assembled pore structure, which is dependent on the monomer composition of a polymer along with its molecular weight and the technique parameters (distance from the nozzle, volume, and polymer concentration in working solution). Their pore morphologies were evaluated and their hydrophobicity was examined. Mesenchymal stem cells (MSCs) isolated from bone marrow were cultivated on a porous film surface. MSCs' attachment differed markedly depending on surface morphology. On strip-formed stamp films, MSCs elongated along the structure, however, they interacted with a larger area of film surface. The honeycomb films and column type films did not set the direction of extrusion, but cell flattening depended on structure topography. Thus, stem cells can "feel" the various surface morphologies of self-assembled honeycomb films and change their behavior depending on it. |
