Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPARγ.

Autor:	Ferraz Carbonel AA; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Federal University of São Paulo (EPM/UNIFESP), 740 Edifício Lemos Torres-2° andar, Vila Clementino, São Paulo 04023-900, SP, Brazil.; Department of Gynecology, Paulista School of Medicine, Federal University of São Paulo (EPM/UNIFESP), São Paulo 04023-900, SP, Brazil., da Silva RA; Biosciences Graduate Program, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University (IBILCE/UNESP), São José do Rio Preto 15054-000, SP, Brazil., de Souza Ferreira LP; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Federal University of São Paulo (EPM/UNIFESP), 740 Edifício Lemos Torres-2° andar, Vila Clementino, São Paulo 04023-900, SP, Brazil., Vieira RR; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Federal University of São Paulo (EPM/UNIFESP), 740 Edifício Lemos Torres-2° andar, Vila Clementino, São Paulo 04023-900, SP, Brazil., Dos Santos Simões R; Department of Obstetrics and Gynecology, Medicine Faculty of University of São Paulo (FMUSP), São Paulo 05403-911, SP, Brazil., da Silva Sasso GR; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Federal University of São Paulo (EPM/UNIFESP), 740 Edifício Lemos Torres-2° andar, Vila Clementino, São Paulo 04023-900, SP, Brazil., de Jesus Simões M; Structural and Functional Biology Graduate Program, Paulista School of Medicine, Federal University of São Paulo (EPM/UNIFESP), 740 Edifício Lemos Torres-2° andar, Vila Clementino, São Paulo 04023-900, SP, Brazil.; Department of Gynecology, Paulista School of Medicine, Federal University of São Paulo (EPM/UNIFESP), São Paulo 04023-900, SP, Brazil., Soares Junior JM; Department of Obstetrics and Gynecology, Medicine Faculty of University of São Paulo (FMUSP), São Paulo 05403-911, SP, Brazil., Azevedo Lima PD; Queen's Cardiopulmonary Unit (QCPU), Queen's University, Kingston, ON K7L 3J9, Canada., Borges FT; Department of Medicine, Nephrology Division, Paulista School of Medicine, Federal University of São Paulo (EPM/UNIFESP), São Paulo 04023-900, SP, Brazil.; Interdisciplinary Graduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo 01506-000, SP, Brazil.
Jazyk:	angličtina
Zdroj:	Nutrients [Nutrients] 2022 Jun 21; Vol. 14 (13). Date of Electronic Publication: 2022 Jun 21.
DOI:	10.3390/nu14132567
Abstrakt:	Diabetes associated with post-menopause is related to a worse condition of kidney disease. Taking into consideration that this disorder may be regulated by estrogenic mediators, we evaluated the renal protective effect of isoflavone. We investigated the role of the PPARγ in the pathogenesis of the disease. For this study, we used diabetic female rats in a postmenopausal model through ovariectomy. The animals were treated with isoflavone or 17β-estradiol. A dosage was administered to bring on blood glycemia, and through immunohistochemistry, we evaluated the immunoreactivity of PPARγ in the endometrium and renal tissue. We analyzed the immunoreactivity of renal injury molecule KIM-1 and the collagen and glycogen densities in the kidney. Through bioinformatics analysis, we observed PPARγ and COL1A1 gene expression under the influence of different glucose doses. In particular, we observed that isoflavone and 17β-estradiol regulate blood glycemia. Renal injury was inhibited by isoflavone, observed by a reduction in KIM-1, along with glycogen accumulation. These benefits of isoflavone may be associated with PPARγ overexpression in the kidneys and endometrium of diabetic ovariectomized rats.
Databáze:	MEDLINE
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