| Autor: |
Al-Khayri JM; Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia., Mahdy EMB; National Gene Bank (NGB), Agricultural Research Centre (ARC), Giza 12613, Egypt., Taha HSA; Genetics Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt., Eldomiaty AS; Genetics Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt., Abd-Elfattah MA; Pomology Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt., Abdel Latef AAH; Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena 83523, Egypt., Rezk AA; Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia., Shehata WF; Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia., Almaghasla MI; Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia.; Plant Pests, and Diseases Unit, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia., Shalaby TA; Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia.; Horticulture Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt., Sattar MN; Central Laboratories, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia., Ghazzawy HS; Date Palm Research Center of Excellence, King Faisal University, Al-Ahsa 31982, Saudi Arabia., Awad MF; Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Alali KM; Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia., Jain SM; Department of Agricultural Sciences, University of Helsinki, 00014 Helsinki, Finland., Hassanin AA; Genetics Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt. |
| Abstrakt: |
Determining the appropriate parents for breeding programs is the most important decision that plant breeders must make to maximize the genetic variability and produce excellent recombinant genotypes. Several methods are used to identify genotypes with desirable phenotypic features for breeding experiments. In this study, five kalanchoe genotypes were morphologically characterized by assessing plant height, number of inflorescences, number of flowers, flower length, flower diameter and number of petals. The analysis showed the distinction of yellow kalanchoe in the plant height trait, while the orange kalanchoe was distinguished in the number of inflorescences, the number of flowers and flower length traits, whereas the violet kalanchoe possessed the largest flower diameter and the highest number of petals. The molecular profiling was performed by random amplified polymorphism DNA (RAPD), inter-simple sequence repeats (ISSR) and start codon targeted (SCoT)-polymerase chain reaction (PCR) tools. Genomic DNA was extracted from young leaves and the PCR reactions were performed using ten primers for each SCoT, ISSR and RAPD marker. Only four out of ten primers showed amplicon profiles in all PCR markers. A total of 70 bands were generated by SCoT, ISSR and RAPD-PCR with 35 polymorphic bands and 35 monomorphic bands. The total number of bands of RAPD, ISSR and SCoT was 15, 17 and 38, respectively. The polymorphism percentages achieved by RAPD, ISSR and SCoT were 60.25%, 15% and 57%, respectively. The cluster analysis based on morphological data revealed two clusters. Cluster I consisted of violet and orange kalanchoe, and cluster II comprised red, yellow and purple kalanchoe. Whereas the cluster analysis based on molecular data revealed three clusters. Cluster I included only yellow kalanchoe, cluster II comprised orange and violet kalanchoe while cluster III comprised red, and purple kalanchoe. The study concluded that orange, violet and yellow kalanchoe are distinguished parents for breeding economically valued traits in kalanchoe. Also, the study concluded that SCoT and RAPD markers reproduced reliable banding patterns to assess the genetic polymorphism among kalanchoe genotypes that consider the basis stone for genetic improvements in ornamental plants. |
