| Autor: |
Haftek M; UMR5305, Laboratory of Tissue Biology and Therapeutic Engineering, CNRS and University of Lyon, 8 Avenue Rockefeller, F-69373 Lyon, France., Oji V; Department of Dermatology, University Hospital of Muenster, D-48149 Muenster, Germany., Feldmeyer L; Department of Dermatology, University Hospital Lausanne, CH-1011 Lausanne, Switzerland., Hohl D; Department of Dermatology, University Hospital Lausanne, CH-1011 Lausanne, Switzerland., Hadj-Rabia S; Department of Dermatology, Reference Center for Genodermatoses and Rare Skin Diseases (MAGEC), Institut Imagine, Necker-Enfants Malades Hospital, AP-HP5, University Paris-Centre, F-75015 Paris, France., Abdayem R; UMR5305, Laboratory of Tissue Biology and Therapeutic Engineering, CNRS and University of Lyon, 8 Avenue Rockefeller, F-69373 Lyon, France. |
| Abstrakt: |
We evaluated the presence of tight junction (TJ) remnants in the stratum corneum (SC) of in vitro reconstructed human epidermis and human skin explants subjected or not to an aggressive topical treatment with beta-lipohydroxy salicylic acid (LSA) for 24 h. LSA-treated samples showed an increased presence of TJ remnants in the two lowermost layers of the SC, as quantified with standard electron microscopy. The topical aggression-induced overexpression of TJ-like cell-cell envelope fusions may influence SC functions: (1) directly, through an enhanced cohesion, and (2) indirectly, by impeding accessibility of peripheral corneodesmosomes to extracellular hydrolytic enzymes and, thus, slowing down desquamation. Observations of ichthyotic epidermis in peeling skin disease (PSD; corneodesmosin deficiency; two cases) and ichthyosis hypotrichosis sclerosing cholangitis syndrome (IHSC/NISCH; absence of claudin-1; two cases) also demonstrated increased persistence of TJ-like intercellular fusions in pathological SC and contributed to the interpretation of the diseases' pathological mechanisms. |
