Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture.

Autor: Nogueira LFB; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil.; Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy., Eufrásio Cruz MA; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil., Aguilar GJ; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil., Tapia-Blácido DR; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil., da Silva Ferreira ME; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto 14040-900, Brazil., Maniglia BC; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil., Bottini M; Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy., Ciancaglini P; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil., Ramos AP; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Jun 30; Vol. 23 (13). Date of Electronic Publication: 2022 Jun 30.
DOI: 10.3390/ijms23137277
Abstrakt: Inspired by the composition and confined environment provided by collagen fibrils during bone formation, this study aimed to compare two different strategies to synthesize bioactive hybrid membranes and to assess the role the organic matrix plays as physical confinement during mineral phase deposition. The hybrid membranes were prepared by (1) incorporating calcium phosphate in a biopolymeric membrane for in situ hydroxyapatite (HAp) precipitation in the interstices of the biopolymeric membrane as a confined environment (Methodology 1) or (2) adding synthetic HAp nanoparticles (SHAp) to the freshly prepared biopolymeric membrane (Methodology 2). The biopolymeric membranes were based on hydrolyzed collagen (HC) and chitosan (Cht) or κ-carrageenan (κ-carr). The hybrid membranes presented homogeneous and continuous dispersion of the mineral particles embedded in the biopolymeric membrane interstices and enhanced mechanical properties. The importance of the confined spaces in biomineralization was confirmed by controlled biomimetic HAp precipitation via Methodology 1. HAp precipitation after immersion in simulated body fluid attested that the hybrid membranes were bioactive. Hybrid membranes containing Cht were not toxic to the osteoblasts. Hybrid membranes added with silver nanoparticles (AgNPs) displayed antibacterial action against different clinically important pathogenic microorganisms. Overall, these results open simple and promising pathways to develop a new generation of bioactive hybrid membranes with controllable degradation rates and antimicrobial properties.
Databáze: MEDLINE
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