Mucosal Plasma Cell Activation and Proximity to Nerve Fibres Are Associated with Glycocalyx Reduction in Diarrhoea-Predominant Irritable Bowel Syndrome: Jejunal Barrier Alterations Underlying Clinical Manifestations.

Autor: Pardo-Camacho C; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Ganda Mall JP; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Department of Biomedical and Clinical Sciences, Linköping University, 58185 Linköping, Sweden., Martínez C; Vascular and Renal Translational Research Group, Lleida Institute for Biomedical Research Dr. Pifarré. Foundation (IRBLleida), Av. Alcalde Rovira Roure 80, 25198 Lleida, Spain., Pigrau M; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain., Expósito E; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Albert-Bayo M; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Melón-Ardanaz E; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Nieto A; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Department of Gastroenterology, Vall d'Hebron Hospital Universitari, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Rodiño-Janeiro B; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Fortea M; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Guagnozzi D; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Department of Gastroenterology, Vall d'Hebron Hospital Universitari, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Rodriguez-Urrutia A; Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Department of Mental Health, Vall d'Hebron Hospital Universitari, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Instituto de Salud Carlos III, 28029 Madrid, Spain., Torres I; Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Department of Pathology, Hospital Universitari Vall d'Hebrón, Passeig Vall d'Hebron 119-129, 08035 Bar celona, Spain., Santos-Briones I; Facultat Ciències de la Salut, Universitat Ramon LLull-Blanquerna, C/Padilla 326, 08025 Barcelona, Spain., Azpiroz F; Department of Gastroenterology, Vall d'Hebron Hospital Universitari, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, 28029 Madrid, Spain., Lobo B; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Department of Gastroenterology, Vall d'Hebron Hospital Universitari, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Alonso-Cotoner C; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Department of Gastroenterology, Vall d'Hebron Hospital Universitari, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, 28029 Madrid, Spain., Santos J; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.; Department of Gastroenterology, Vall d'Hebron Hospital Universitari, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, 28029 Madrid, Spain., González-Castro AM; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Laboratory of Neuro-Immuno-Gastroenterology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Vicario M; Laboratory of Translational Mucosal Immunology, Digestive System Research Unit, Vall d'Hebron Institut de Recerca, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.; Department of Gastrointestinal Health, Nestlé Institute of Health Sciences, Société des Produits Nestlé S.A., Nestlé Research, Vers-chez-les-Blanc, 1000 Lausanne, Switzerland.
Jazyk: angličtina
Zdroj: Cells [Cells] 2022 Jun 28; Vol. 11 (13). Date of Electronic Publication: 2022 Jun 28.
DOI: 10.3390/cells11132046
Abstrakt: Irritable bowel syndrome (IBS) is a disorder of brain-gut interaction characterised by abdominal pain and changes in bowel habits. In the diarrhoea subtype (IBS-D), altered epithelial barrier and mucosal immune activation are associated with clinical manifestations. We aimed to further evaluate plasma cells and epithelial integrity to gain understanding of IBS-D pathophysiology. One mucosal jejunal biopsy and one stool sample were obtained from healthy controls and IBS-D patients. Gastrointestinal symptoms, stress, and depression scores were recorded. In the jejunal mucosa, RNAseq and gene set enrichment analyses were performed. A morphometric analysis by electron microscopy quantified plasma cell activation and proximity to enteric nerves and glycocalyx thickness. Immunoglobulins concentration was assessed in the stool. IBS-D patients showed differential expression of humoral pathways compared to controls. Activation and proximity of plasma cells to nerves and IgG concentration were also higher in IBS-D. Glycocalyx thickness was lower in IBS-D compared to controls, and this reduction correlated with plasma cell activation, proximity to nerves, and clinical symptoms. These results support humoral activity and loss of epithelial integrity as important contributors to gut dysfunction and clinical manifestations in IBS-D. Additional studies are needed to identify the triggers of these alterations to better define IBS-D pathophysiology.
Databáze: MEDLINE
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