Effect of Select Tannin Sources on Pathogen Control and Microbial Nitrogen Metabolism in Composted Poultry Litter Intended for Use as a Ruminant Crude Protein Feedstuff.

Autor: Arzola-Alvarez C; College of Animal Science and Ecology, Autonomous University of Chihuahua, Chihuahua, Mexico., Anderson RC; United States Department of Agriculture, Agricultural Research Service, Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, College Station, TX, United States., Hume ME; United States Department of Agriculture, Agricultural Research Service, Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, College Station, TX, United States., Ledezma E; College of Animal Science and Ecology, Autonomous University of Chihuahua, Chihuahua, Mexico., Ruiz-Barrera O; College of Animal Science and Ecology, Autonomous University of Chihuahua, Chihuahua, Mexico., Castillo-Castillo Y; College of Animal Science and Ecology, Autonomous University of Chihuahua, Chihuahua, Mexico., Arzola-Rubio A; College of Animal Science and Ecology, Autonomous University of Chihuahua, Chihuahua, Mexico., Ontiveros-Magadan M; College of Animal Science and Ecology, Autonomous University of Chihuahua, Chihuahua, Mexico., Min BR; Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL, United States., Wottlin LR; United States Department of Agriculture, Agricultural Research Service, Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, College Station, TX, United States., Copado R; College of Veterinary Medicine, Autonomous University of Nuevo Leon, Monterrey Nuevo Leon, Mexico., Salinas-Chavira J; College of Veterinary Medicine, Universidad Autónoma de Tamaulipas, Matamoros, Mexico.
Jazyk: angličtina
Zdroj: Frontiers in veterinary science [Front Vet Sci] 2022 Jun 21; Vol. 9, pp. 930980. Date of Electronic Publication: 2022 Jun 21 (Print Publication: 2022).
DOI: 10.3389/fvets.2022.930980
Abstrakt: Poultry litter is a good crude protein supplement for ruminants but must be treated to kill pathogens before feeding. Composting effectively kills pathogens but risks loss of ammonia due to uric acid degradation. The objectives of this study were to test the ability of tannins to reduce pathogens and preserve uric acid during poultry litter composting. In two experiments, poultry litter was mixed with phosphate buffer and distributed to 50-ml tubes (three tubes/treatment per sample day) amended with 1 ml buffer alone or buffer containing pine bark, quebracho, chestnut, or mimosa tannins. Treatments achieved 0.63% (wt/wt) quebracho, chestnut, or mimosa tannins in experiment 1, or 4.5% pine bark or 9% quebracho, chestnut, or mimosa tannins in experiment 2. Tubes were inoculated with a novobiocin- and nalidixic acid-resistant Salmonella typhimurium, closed with caps, and incubated at successive 3-day increments at 22, 37, and 42°C, respectively. In experiment 1, bacterial counts in contents collected on days 0, 6, and 9 revealed a treatment by day effect ( p < 0.03), with the Salmonella challenge being 1.3 log 10 CFU/g higher in quebracho-treated composts than in untreated controls after 6 days of composting. After 9 days of composting, Salmonella , wildtype Escherichia coli , and total aerobes in untreated and all tannin-treated composts were decreased by about 2.0 log 10 CFU/g compared to day 0 numbers (3.06, 3.75, and 7.77 log 10 CFU/g, respectively). Urea and ammonia concentrations tended ( p < 0.10) to be increased in chestnut-treated composts compared to controls and concentrations of uric acid, urea, and ammonia were higher ( p < 0.05) after 9 days of composting than on day 0. Despite higher tannin application in experiment 2, antibacterial effects of treatment or day of composting were not observed ( p > 0.05). However, treatment by time of composting interactions was observed ( p < 0.05), with quebracho- and chestnut-treated composts accumulating more uric acid after 24 h and 9 days of composting and chestnut-, mimosa- or quebracho-treated composts accumulating less ammonia than untreated composts. Results demonstrate that composting may effectively control pathogens and that tannin treatment can help preserve the crude protein quality of composting poultry litter.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Arzola-Alvarez, Anderson, Hume, Ledezma, Ruiz-Barrera, Castillo-Castillo, Arzola-Rubio, Ontiveros-Magadan, Min, Wottlin, Copado and Salinas-Chavira.)
Databáze: MEDLINE
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