Effects of end-stage osteoarthritis on markers of skeletal muscle Long INterspersed Element-1 activity.

Autor: Osburn SC; School of Kinesiology, Auburn University, 301 Wire Road, Office 260, Auburn, AL, 36849, USA., Romero MA; Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA, USA., Roberson PA; Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, USA., Mumford PW; School of Health Sciences, Lindenwood University, Saint Charles, MO, USA., Wiggins DA; Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.; UAB Center for Exercise Medicine, University of Alabama at Birmingham, Birmingham, AL, USA., McAdam JS; Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.; UAB Center for Exercise Medicine, University of Alabama at Birmingham, Birmingham, AL, USA., Drummer DJ; Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.; UAB Center for Exercise Medicine, University of Alabama at Birmingham, Birmingham, AL, USA., Bridges SL Jr; Department of Medicine, Hospital for Special Surgery, New York, NY, USA.; Division of Rheumatology, Weill Cornell Medical Center, New York, NY, USA., Bamman MM; Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.; UAB Center for Exercise Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.; Florida Institute for Human and Machine Cognition, Pensacola, FL, USA., Roberts MD; School of Kinesiology, Auburn University, 301 Wire Road, Office 260, Auburn, AL, 36849, USA. mdr0024@auburn.edu.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2022 Jul 07; Vol. 15 (1), pp. 245. Date of Electronic Publication: 2022 Jul 07.
DOI: 10.1186/s13104-022-06113-0
Abstrakt: Objective: Long INterspersed Element-1 (L1) is an autonomous transposable element in the genome. L1 transcripts that are not reverse transcribed back into the genome can accumulate in the cytoplasm and activate an inflammatory response via the cyclic GMP-AMP (cGAS)-STING pathway. We examined skeletal muscle L1 markers as well as STING protein levels in 10 older individuals (63 ± 11 y, BMI = 30.2 ± 6.8 kg/m 2 ) with end-stage osteoarthritis (OA) undergoing total hip (THA, n = 4) or knee (TKA, n = 6) arthroplasty versus 10 young, healthy comparators (Y, 22 ± 2 y, BMI = 23.2 ± 2.5 kg/m 2 ). For OA, muscle was collected from surgical (SX) and contralateral (CTL) sides whereas single vastus lateralis samples were collected from Y.
Results: L1 mRNA was higher in CTL and SX compared to Y (p < 0.001 and p = 0.001, respectively). Protein expression was higher in SX versus Y for ORF1p (p = 0.002) and STING (p = 0.022). While these data are preliminary due to limited n-sizes and the lack of a BMI-matched younger control group, higher L1 mRNA expression, ORF1p and STING protein are evident in older versus younger adults. More research is needed to determine whether cGAS-STING signaling contributes to heightened muscle inflammation during aging and/or OA.
(© 2022. The Author(s).)
Databáze: MEDLINE
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